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磷酸化轉(zhuǎn)錄因子SOX9蛋白抗體說(shuō)明書(shū) 英文名稱  Anti-phospho-SOX9(Ser181)

中文名稱  磷酸化轉(zhuǎn)錄因子SOX9蛋白抗體說(shuō)明書(shū) 

別    名 SOX9(phospho S181); CMD 1; CMD1; CMPD 1; CMPD1; SOX 9; Sox9; SOX9_HUMAN; SRA 1; SRA1 antibody SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal; SRY (sex determining region Y) box 9; SRY (sex determining region Y)-box 9; SRY (sex-determining region Y)-box 9 protein; Transcription factor SOX 9; Transcription factor SOX-9; transcription factor SOX9; campomelic dysplasia autosomal sex reversal.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit

產(chǎn)品類(lèi)型   一抗 磷酸化抗體  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 發(fā)育生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 60kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human SOX9 around the phosphorylation site of Ser181

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化轉(zhuǎn)錄因子SOX9蛋白抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

Function : Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

Subcellular Location : Nucleus (Potential).

DISEASE : Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.

Defects in SOX9 are the cause of 46,XX sex reversal type 2 (SRXX2) [MIM:278850]. SRXX2 is a condition in which male gonads develop in a genetic female (female to male sex reversal).

Similarity : Contains 1 HMG box DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: P48436.1

Sox9是軟骨形成過(guò)程中一個(gè)十分關(guān)鍵的轉(zhuǎn)錄因子，雖然已經(jīng)有研究表明骨形成蛋白2(bone morphogenetic protein 2,BMP2)能誘導(dǎo)Sox9的表達(dá)。

有學(xué)者認(rèn)為；Sox9蛋白很可能與椎間盤(pán)退變有著密切的關(guān)系，Sox9是膠原蛋白合成過(guò)程中的一個(gè)重要的轉(zhuǎn)錄因子，且在軟骨的發(fā)育、成熟過(guò)程中對(duì)膠原蛋白有著正向調(diào)控作用，Sox9蛋白在男性性腺。

實(shí)驗(yàn)流程：

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