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中文名稱(chēng)  Toll樣受體4抗體品牌 

英文名稱(chēng)  Anti-TLR4/CD284

別    名 TLR4; TLR-4; TLR 4; ARMD10; CD284; CD284 antigen; Homolog of Drosophila toll; hTol; Toll (Drosophila) homolog; TOLL; Toll Endotoxin Hyporesponsiveness; Toll like receptor 4; Toll like receptor 4 precursor; TLR4_HUMAN .

產(chǎn)品屬性：

Toll樣受體4抗體品牌 濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg 1ml/1mg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Sheep

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域    心 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞膜受體

蛋白分子量  predicted molecular weight: 90kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TLR4

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Toll樣受體4抗體品牌 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The Toll-like receptor 4 by activating natural immunity, specific immune response involved in the start-up, Toll-like receptor 4 as an important signal transduction transmembrane receptor involved in the toxin-induced inflammation in the pathological process, its mechanisms of control On a growing concern. all regions were either double-stranded or sequenced with an alternatechemistry or covered by high quality data(i.e., phred quality >=30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by at least one subclone; and the assembly was confirmed by restriction digest, except on the rare occasion of the clone being a YAC. The following abbreviations are used to associate primary accession numbers given in the feature table with their source databases: Em:, EMBL; Sw:, SWISSPROT; Tr:, TREMBL; Wp:, WORMPEP; Information on the WORMPEP database can be found at. TLR-4 plays an important role in microvascular leakage and leukocyte adhesion under the inflammatory condition associated with nonseptic thermal injury.

Function : Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

Subcellular Location : Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

Tissue Specificity : Membrane.

Post-translational modifications : N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

DISEASE : Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

Similarity : Belongs to the Toll-like receptor family.

Contains 18 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 TIR domain.

Database links : UniProtKB/Swiss-Prot: O00206.2

Entrez Gene: 7099 Human

Entrez Gene: 21898 Mouse

Entrez Gene: 29260 Rat

SwissProt: O00206 Human

SwissProt: Q9QUK6 Mouse

SwissProt: Q9QX05 Rat

Unigene: 174312 Human

Unigene: 38049 Mouse

Toll樣受體4（TLR4）通過(guò)激活天然免疫,參與特異性免疫應(yīng)答的啟動(dòng), Toll樣受體4(TLR4)作為一種重要跨膜信號(hào)轉(zhuǎn)導(dǎo)受體參與了內(nèi)毒素誘發(fā)反應(yīng)的病理過(guò)程,對(duì)其調(diào)控機(jī)制的研究日益受到關(guān)注.

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優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

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技術(shù)外包服務(wù):

★Toll樣受體4抗體品牌 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

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