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糖皮質激素誘導受體抗體說明書 英文名稱  Anti-TNFRSF18/GITR

中文名稱  糖皮質激素誘導受體抗體說明書 

別    名 Activation inducible TNFR family receptor; Activation-inducible TNFR family receptor; CD357; AITR; GITR; GITR D; GITR-D; GITRD; Glucocorticoid induced TNFR related protein; glucocorticoid-induced tnf receptor ligand; Glucocorticoid induced TNFR related protein; TNF receptor superfamily activation inducible protein; Glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation inducible protein; TNR18_HUMAN; TNFRSF 18; TNFRSF18; Tumor necrosis factor receptor superfamily member 18; Tumor necrosis factor receptor superfamily member 18 precursor.

產品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat

產品類型   一抗  

研究領域     細胞生物 免疫學 生長因子和激素 t-淋巴細胞

蛋白分子量  predicted molecular weight: 24kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TNFRSF18

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

糖皮質激素誘導受體抗體說明書 產品應用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品介紹 TNFRSF18 or GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18) originally identified in the mouse by comparing untreated and dexamethasone-treated murine T cell hybridoma cells. Human GITR was subsequently identified by searching an expressed sequence tag database. GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR appears to be highly expressed on CD4+CD25+ T regulatory cells and has been shown to induce NF-kB activation through TRAF2/NIK signaling. GITR has been proposed to be involved in the development of regulatory T cells and to regulate the activity of Th1 subsets. GITR binds GITRL, a TNF superfamily ligand expressed on endothelial cells, dendritic cells, macrophages, and B cells.

Function : Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Subunit : Binds to TRAF1, TRAF2, and TRAF3, but not TRAF5 and TRAF6. Binds through its C-terminus to SIVA1/SIVA.

Subcellular Location : Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Secreted.

Tissue Specificity : Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.

Similarity : Contains 3 TNFR-Cys repeats.

Database links : UniProtKB/Swiss-Prot: Q9Y5U5.1

GITR屬于受體超家族（TNFRSF），GITR參與信號，在T細胞的活化、凋亡,尤其是阻斷調節(jié)性T細胞（T regulatory cell）的等活動中發(fā)揮主要作用。

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