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全、新、優(yōu)、品、好四大特點(diǎn)：

Patched/PTCH抗體規(guī)格 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線(xiàn)技術(shù)咨詢(xún)。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂(yōu)。

Patched/PTCH抗體規(guī)格 英文名稱(chēng)  Anti-Patched/PTCH

中文名稱(chēng)  Patched/PTCH抗體規(guī)格 

別    名 PTC1; A230106A15Rik; BCNS; FLJ26746; FLJ42602; Holoprosencephaly 7; HPE7; mes; NBCCS; OTTHUMP00000021709; OTTHUMP00000021710; Patched; Patched (Drosophila) homolog; Patched 1; Patched homolog (Drosophila); Patched homolog 1 (Drosophila); Patched homolog 1; Patched protein homolog 1; PTC; PTC1; PTCH; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH protein; PTCH1; PTCH1 protein; PTCH11; Ptch2.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Cow, Horse, Rabbit

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域     細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 161kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Patched/PTCH N-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Patched/PTCH抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.

Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.

Ptch蛋白是細(xì)胞表面接受Hh信號(hào)蛋白的受體，目前主要用于方面的研究。

實(shí)驗(yàn)流程：

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CL-0040BT-474(人導(dǎo)管癌細(xì)胞)5×106cells/瓶×2

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青霉素/鏈霉素溶液P/S

Hep 3B2.1-7[Hep 3B;Hep-3B;Hep3B]細(xì)胞，人細(xì)胞 人胚腎細(xì)胞(亞系克隆),FIP293細(xì)胞 胎兒細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

大耳山羊皮膚細(xì)胞;LDG-3

SERPIND1 Others Human 人 SerpinD1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0039BSC-1(猴腎細(xì)胞)5×106cells/瓶×2

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小鼠腸巨噬細(xì)胞完全培養(yǎng)基 100mL

RM-1小鼠細(xì)胞 RM-1 mouse prostate cancer cells RPMI-1640(GIBCO)+10%FBS

IL10 Protein Sus scrofa (Pig) 重組野豬 IL10 / Ierleukin-10 蛋白

SK-MEL-1(人皮膚細(xì)胞) 5×106cells/瓶×2 V79(中國(guó)倉(cāng)鼠肺細(xì)胞)

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技術(shù)外包服務(wù):

★Patched/PTCH抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。