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突觸后密度蛋白95抗體說明書 英文名稱  Anti-PSD95/post-synaptic density protein 95

中文名稱  突觸后密度蛋白95抗體說明書 

別    名 Discs large homolog 4; DLG 4; DLG4; Human post-synaptic density protein 95; Postsynaptic density protein 95; PSD 95; PSD-95; SAP 90; SAP90; Synapse associated protein 90.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit

產(chǎn)品類型   一抗  

研究領域    細胞生物 免疫學 神經(jīng)生物學

蛋白分子量  predicted molecular weight: 80kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PSD95 C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

突觸后密度蛋白95抗體說明書 產(chǎn)品應用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Synaptic Marker

postsynaptic density protein 95 is a molecular scaffolding protein that binds and clusters N-methyl-D-aspartate receptors at neuronal synapses; may be involved in guanine nucleotide-mediated signal transduction pathway. This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with DLG2. With DLG2 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins.

Subcellular Location : Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell junction, synapse, synaptosome. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.

Tissue Specificity : Brain.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

Contains 1 SH3 domain.

該蛋白質(zhì)PSD95能夠幫助建立神經(jīng)突觸的架構(gòu)，并且促成突觸其他部分的成熟，例如加強目標細胞上的glutamate受體的聚集、增加樹突棘（dendritic spines）的數(shù)量和大小以及增加神經(jīng)傳導物質(zhì)glutamate (谷氨酸鹽)釋放的量。這種蛋白質(zhì)與阿滋海默癥有關。

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技術(shù)外包服務:

★突觸后密度蛋白95抗體說明書 分子生物學：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等

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★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構(gòu)建、細胞RNAi技術(shù)等等。