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抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 凋亡相關(guān)蛋白6抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購(gòu)信息：
英文名稱(chēng)  Anti-PDCD6/ALG2 

中文名稱(chēng)   凋亡相關(guān)蛋白6抗體規(guī)格 

別    名  ALG 2; ALG2; Apoptosis linked gene 2; Apoptosis linked gene 2 protein; Apoptosis-linked gene 2 protein; MA 3; MA3; MGC111017; MGC119050; MGC9123; PDCD 6; Pdcd6; PDCD6_HUMAN; PEF 1B; PEF1B; Probable calcium binding protein ALG 2; Probable calcium binding protein ALG2; Probable calcium-binding protein ALG-2; Programmed cell death 6; Programmed cell death protein 6; PS 2; PS2.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域    細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白

蛋白分子量  predicted molecular weight: 22kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PDCD6 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

凋亡相關(guān)蛋白6抗體規(guī)格 產(chǎn)品應(yīng)用    ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5. [provided by RefSeq, May 2012].

Function : Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity.

Subunit : Self-associates (By similarity). Homodimer. Interacts with PEF1. Interacts with RBM22. Interacts with PLSCR4, ANXA7 and TSG101. Interacts with SEC31A, PDCD6IP, and the N-termini of ANXA11 and PLSCR3 in a calcium-dependent manner. Interacts with DAPK1.

Subcellular Location : Nucleus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus. Note=Interaction with RBM22 induces relocalization from the cytoplasm to the nucleus. Translocated from the cytoplasm to the nucleus after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules.

Database links : UniProtKB/Swiss-Prot: O75340.1

抗體的鑒定：

1）凋亡相關(guān)蛋白6抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線(xiàn),計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

Anti-CPT1A/FITC 熒光素標(biāo)記抗肉毒堿棕櫚?；D(zhuǎn)移酶1A抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-KLK3/FITC 熒光素標(biāo)記激肽釋放酶3/舒素3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CCDC23 卷曲螺旋結(jié)構(gòu)域蛋白23抗體 規(guī)格 0.2ml

H1N1(Influenza A virus ,hemagglutinin) 病毒A抗原 0.5mg

HOXD9 英文名稱(chēng)： 轉(zhuǎn)錄調(diào)節(jié)因子HOXD9抗體 0.1ml

Rhesus antibody Rh SMARCA6/HELLS 淋巴特異性解旋酶抗體 規(guī)格 0.2ml

Anti-KLK3/FITC 熒光素標(biāo)記激肽釋放酶3/舒素3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

C I CP ELISA Kit 大鼠Ⅰ型前膠原C末端肽Multi-class antibodies規(guī)格： 48T

Anti-Phospho-YAP1 (Ser127) 磷酸化原癌基因Yes相關(guān)蛋白1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh G protein beta subunit GI G蛋白/鳥(niǎo)苷酸結(jié)合蛋白抗體 規(guī)格 0.2ml

mALB ELISA Kit 大鼠微量蛋白 96T

Alpha-synuclein (nitro-Tyr39) 英文名稱(chēng)： 硝基化α-突觸核蛋白 0.1ml

ARFGAP3 英文名稱(chēng)： ADP核糖基化因子GTP酶激活蛋白1抗體 0.2ml

Anti-Phospho-YAP1 (Ser127) 磷酸化原癌基因Yes相關(guān)蛋白1抗體Multi-class antibodies規(guī)格： 0.1ml

Alpha2-MG(Human Alpha2 macroglobulin) ELISA Kit 人α2巨球蛋白Multi-class antibodies規(guī)格： 48T

Anti-p38MAPK/MAPK14 原活化蛋白激酶p38抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh human Fibrinogen Monoclonal 人纖維蛋白原單克隆抗體 規(guī)格 0.2ml

Human anti-Ku-antibody ELISA Kit 人抗Ku抗體 96T

Phospho-PPAR Gamma (ser273) 英文名稱(chēng)： 磷酸化過(guò)氧化酶活化增生受體γ抗體 PPARγ 0.1ml

CCDC38 英文名稱(chēng)： 卷曲螺旋結(jié)構(gòu)域蛋白38抗體 0.2ml

Anti-p38MAPK/MAPK14 原活化蛋白激酶p38抗體Multi-class antibodies規(guī)格： 0.1ml

CL-0406NCI-H838(人非小細(xì)胞細(xì)胞)5×106cells/瓶×2

ANGPTL1 Others Canine 狗 ANGPTL1 / Angiopoietin-like 1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人星形膠質(zhì)細(xì)胞RNAHA miRNA5 μg

家兔皮膚細(xì)胞;DR-S1 神經(jīng)母細(xì)胞瘤細(xì)胞，SH-SY5Y細(xì)胞 U14細(xì)胞，小鼠子細(xì)胞

人類(lèi)成纖維細(xì)胞系;CNLMG-B5538SKIN

HA Others H1N1 甲型 H1N1 (A/Solomon Islands/3/2006) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0405NCI-H716(人結(jié)直腸腺癌細(xì)胞)5×106cells/瓶×2

EPHA3 Others Mouse 小鼠 EphA3 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人臍平滑肌細(xì)胞總RNAHUVSMC NA

家兔骨髓間充質(zhì)干細(xì)胞;2012083MSC 人主動(dòng)脈平滑肌細(xì)胞，T/G HA-VSMC細(xì)胞 MPC-11細(xì)胞，小鼠漿細(xì)胞瘤

人胚;MRC-5

HA Others H4N6 甲型 H4N6 (A/Swine/Oario/01911-1/99) 血凝素 (Hemagglutinin / HA) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

凋亡相關(guān)蛋白6抗體規(guī)格 人肝細(xì)胞 (HH) (1×106 )

敘利亞倉(cāng)鼠腎細(xì)胞;BHK-21

CL-0051CaES-17(人細(xì)胞)5×106cells/瓶×2

小鼠胚胎成纖維細(xì)胞(來(lái)自NIH3T3);PA12 小鼠肺平滑肌細(xì)胞完全培養(yǎng)基 100mL

HCT/Taxol 人紫杉醇耐藥

ACE2 Others Mouse 小鼠 ACE2 / ACEH 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)