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蛋白酪氨酸磷酸酶PTPσ抗體說(shuō)明書 英文名稱  Anti-PTPRS

中文名稱  蛋白酪氨酸磷酸酶PTPσ抗體說(shuō)明書 

別    名 Protein tyrosine phosphatase PTPsigma; Protein tyrosine phosphatase receptor type S; Protein tyrosine phosphatase receptor type sigma; Ptprs; PTPRS_HUMAN; PTPSIGMA; R PTP sigma; R-PTP-S; R-PTP-sigma; Receptor-type tyrosine-protein phosphatase S; Receptor-type tyrosine-protein phosphatase sigma.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 214kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PTPRS

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

蛋白酪氨酸磷酸酶PTPσ抗體說(shuō)明書 產(chǎn)品應(yīng)用   ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Protein tyrosine phosphatases, or PTPs, are type I transmembrane proteins, membrane associated proteins or proteins localized in nuclei. Examples of transmembrane PTPs are LAR, PTP alpha, PTP beta, PTP gamma, PTP delta, PTP epsilon, PTP zeta, PTP thet, PTP upsilon and PTPs. Transmembrane PTPsigma play diverse roles during development and in adult tissues. Immunodepletion studies have suggested LAR to be a regulator of insulin receptor phosphorylation. PTP alpha activity is increased twofold in response to phorbol ester stimulation, resulting in serine phosphorylation either directly or indirectly by members of the PKC family. Overexpression of v-H-Ras and Neu, but not Myc or Int2, in mammary tumors has been shown to induce PTPe expression. An alternative splicing event leads to a nervous tissue-specific chondroitin sulfate proteoglycan called phosphacan, which represents the amino terminal portion of PTP omega. PTP thet and PTP?share a conserved amino terminal 160 amino acid MAM domain which facilitates homophilic binding. PTP upsilon localizes to points of cell contact and may be involved in regulating the assembly and disassembly of cadherin/catenin complexes in vivo. PTPsigma contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. PTPsigma may also be involved in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.

Function : Interacts with LAR-interacting protein LIP.1.

Subunit : Interacts with PPFIA1, PPFIA2 and PPFIA3.

Subcellular Location : Membrane; Single-pass type I membrane protein.

Post-translational modifications : A cleavage occurs, separating the extracellular domain from the transmembrane segment. This process called 'ectodomain shedding' is thought to be involved in receptor desensitization, signal transduction and/or membrane localization.

Similarity : Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.

Contains 8 fibronectin type-III domains.

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 2 tyrosine-protein phosphatase domains.

Database links : UniProtKB/Swiss-Prot: Q13332.3

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