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產(chǎn)品訂購信息：
英文名稱  Anti-PQBP1 

中文名稱   多谷氨酰胺結(jié)合蛋白1抗體品牌 

別    名  38 kDa nuclear protein containing a WW domain; Mental retardation, X linked 55; MRX55; MRXS3; MRXS8; Npw38; Nuclear protein containing WW domain 38 kD; Polyglutamine binding protein 1; Polyglutamine tract binding protein 1; Polyglutamine tract-binding protein 1; Polyglutamine-binding protein 1; PQBP 1; PQBP-1; PQBP1; PQBP1_HUMAN; RENS1; SHS; Sutherland Haan X linked mental retardation syndrome.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 神經(jīng)生物學(xué) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 30kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PQBP1 (185-265aa) 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

多谷氨酰胺結(jié)合蛋白1抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Polyglutamine(Q) tract binding protein-1 (PQBP-1) is a transcription repressor that associates with polyglutamine tract-containing transcription regulators and causative genes for neurodegenerative disorders. Hepta- and di-amino acid repeat sequences rich in polar residues are essential for PQBP-1 to interact with polyglutamine tract-containing proteins (i.e. huntingtin, androgen receptor and Brain-2). PQBP-1 contains a WWP/WW domain that binds proline-rich motifs and a C2 domain that can influence Ca2+-dependent phospholipid signaling. PQBP-1 localizes to the nucleus and is present in neurons throughout the brain, with abundant levels in hippocampus, cerebellar cortex and olfactory bulb. The human PQBP-1 gene maps to chromosome Xp11.23.

Function : May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery. May be involved in ATXN1 mutant-induced cell death. The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit.

Subunit : nteracts with POU3F2/Brn-2, ATXN1, TXNL4A, HTT and AR.Interaction with ATXN1 correlates positively with the length of thepolyglutamine tract. Interacts with RNA polymerase II large subunitin a phosphorylation-dependent manner. Forms a ternary complex withATXN1 mutant and phosphorylated RNA polymerase II.

Subcellular Location : Nucleus. Co-localized with POU3F2. Co-localized with ATXN1 in nuclear inclusion bodies.

DISEASE : Defects in PQBP1 are the cause of Renpenning syndrome 1 (RENS1) [MIM:309500]; also known as Sutherland-Haan X-linked mental retardation syndrome (SHS) or X-linked mental retardation syndromes MRXS3/MRXS8/MRX55. The clinical features are mental retardation, microcephaly, short stature, and small testes. The craniofacies tends to be narrow and tall with upslanting palpebral fissures, abnormal nasal configuration, cupped ears, and short philtrum. The nose may appear long or bulbous, with overhanging columella. Less consistent manifestations include ocular colobomas, cardiac malformations, cleft palate, and anal anomalies. RENS1 is more frequently in males than in females where little or no expression is found.

Similarity : Contains 1 WW domain.

Database links : UniProtKB/Swiss-Prot: O60828.1

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PTP/PTPase/CD148(Human protein tyrosine phosphatase) ELISA Kit 人蛋白酪酸磷酸酶 96T

RIT1 英文名稱： RIT1蛋白抗體 0.2ml

CEP290 英文名稱： 中心體蛋白290抗體 0.1ml

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AP(Human Aprotinin) ELISA Kit 人抑肽酶Multi-class antibodies規(guī)格： 48T

Anti-CHRNA2(neuronal) 型乙酰膽堿受體A2(神經(jīng)型)抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh IL-9 白介素9抗體 規(guī)格 0.1ml

BF ELISA Kit 大鼠B因子 96T

PMP22 英文名稱： 外周髓鞘蛋白-22抗體 0.1ml

CEACAM7 英文名稱： 癌胚抗原相關(guān)細(xì)胞粘附分子7抗體 0.1ml

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Anti-Midnolin isoform Protein 2/FITC 熒光素標(biāo)記中腦核仁蛋白2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

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Rabbit Anti-Avidin/PE-CY5 PE-CY5標(biāo)記的兔抗親和素 0.1ml

GIPC1 英文名稱： 樣生長因子1受體相互作用蛋白1抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-Avidin/AP 堿性磷酸酶(AP)標(biāo)記的兔抗親和素 規(guī)格 0.1ml

Anti-TPH /FITC 熒光素標(biāo)記色氨酸羥化酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

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CYTOCHROMEC蛋白表達(dá)西方雜交分析試劑盒5次

Humanmucosaeassociatedepitheliachemokine,MECELISA試劑盒人粘膜相關(guān)上皮趨化因子(MEC/CCL28)ELISA試劑盒規(guī)格：96T/48T

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多谷氨酰胺結(jié)合蛋白1抗體品牌 大鼠成纖維細(xì)胞生長因子23(FGF-23)ELISA試劑盒 ，英文名： FGF-23 ELISA Kit

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抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 多谷氨酰胺結(jié)合蛋白1抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）多谷氨酰胺結(jié)合蛋白1抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。