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產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-PTOP 

中文名稱   腎上腺皮質(zhì)發(fā)育異常同源蛋白抗體規(guī)格 

別    名  PIP1; Acd; ACD_HUMAN; Adrenocortical dysplasia protein homolog; POT1 and TIN2 organizing protein; POT1 and TIN2-interacting protein; TIN2 interacting protein 1; TINT1; TPP1.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 細(xì)胞周期蛋白 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 58kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PTOP 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

腎上腺皮質(zhì)發(fā)育異常同源蛋白抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a protein that is involved in telomerefunction. This protein is one of six core proteins in thetelosome/shelterin telomeric complex, which functions to maintaintelomere length and to protect telomere ends. Through itsinteraction with other components, this protein plays a key role inthe assembly and stabilization of this complex, and it mediates theaccess of telomerase to the telomere. Multiple transcript variantsencoding different isoforms have been found for this gene. Thisgene, which is also referred to as TPP1, is distinct from theunrelated TPP1 gene on chromosome 11, which encodestripeptidyl-peptidase I.

Function : Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity. Plays a role in shelterin complex assembly. May play a role in organogenesis.

Subunit : Component of the shelterin complex (telosome) composed ofTERF1, TERF2, TINF2, TERF2IP ACD and POT1. Forms heterodimers withPOT1. Identified in a complex with POT1 and single-strandedtelomeric DNA. Interacts with STN1/OBFC1 and TINF2.

Subcellular Location : Nucleus. Chromosome, telomere.

MIF(Human Macrophage Migration Inhibitory Factor) ELISA Kit 人巨噬細(xì)胞移動(dòng)抑制因子Multi-class antibodies規(guī)格： 48T

Anti-CXCL9 γ干擾素誘導(dǎo)單核細(xì)胞因子抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Mouse Anti-human IgG 小鼠抗人IgG 規(guī)格 1mg

Ntn1(Human Netrin-1) ELISA Kit 人軸突生長(zhǎng)誘向因子1 96T

RICTOR 英文名稱： 雷帕霉素靶蛋白復(fù)合體2抗體 0.2ml

phospho-c-Jun(Thr91) 英文名稱： 磷酸化原癌基因c-Jun抗體 0.1ml

Anti-CXCL9 γ干擾素誘導(dǎo)單核細(xì)胞因子抗體Multi-class antibodies規(guī)格： 0.2ml

Activin A Human 人激活素 AMulti-class antibodies規(guī)格： 48T

Anti-CK1+5+10+14 高分子量角蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh NR2C/NMDAR2C 谷氨酸受體2C抗體(N端) 規(guī)格 0.1ml

Cover Glass 蓋玻片 24x24mm

TXNDC5 英文名稱： 硫氧還蛋白5抗體 0.2ml

C8orf40 英文名稱： 8號(hào)染色體開放閱讀框40抗體 0.2ml

Anti-CK1+5+10+14 高分子量角蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-Phospho-Mst1 (Thr183)/Mst2 (Thr180) /FITC 熒光素標(biāo)記磷酸化蛋白激酶MST抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Tyk2/Non receptor tyrosine protein kinase 2/FITC 熒光素標(biāo)記非受體酪氨酸蛋白激酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

5-羥色胺受體2A抗體 Anti-5-HTR2A 0.1ml

Mouse Anti-Rabbit IgM/Bio 生物素標(biāo)記的小鼠抗兔IgM 0.1ml

GLB1L3 英文名稱： β半乳糖苷酶1樣蛋白3抗體 0.2ml

Rhesus antibody Rh PSCD1 細(xì)胞附著蛋白PSCD1抗體 規(guī)格 0.1ml

Anti-Tyk2/Non receptor tyrosine protein kinase 2/FITC 熒光素標(biāo)記非受體酪氨酸蛋白激酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠抗胰蛋白酶(AT)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人殺菌肽(cecropin)免疫試劑盒 Human cecropin ELISA Kit

MouseInsulin,INSELISAKit小鼠(INS)ELISA試劑盒規(guī)格：96T/48T

CDK6蛋白表達(dá)西方雜交分析試劑盒5次

HumanMothersagainstdecapeaplegichomolog1,Smad1ELISA試劑盒人Smad1ELISA試劑盒規(guī)格：96T/48T

HumanPolymorphonuclearElastase,PMNElastaseELISAKit人多形核白細(xì)胞彈性蛋白酶(PMNElastase)ELISA試劑盒規(guī)格：96T/48T

大鼠白介素33(IL-33)ELISA試劑盒 ，英文名： IL-33 ELISA Kit

Mouse ierleukin 27 (IL-27) ELISA Kit 小鼠白介素27(IL-27)ELISA試劑盒

ELISA 小鼠高遷移率族蛋白(mouse HMGB1) 48T/96T 進(jìn)口分裝

CLIAKitforINH-BGH(MouseinhibinB)ELISAKit小鼠抑制素B規(guī)格：48T/96T

通用型甘蔗白條病(Xahomonasalbilineans;Xalb)試劑盒20次

ELISAKitiNOS大鼠誘導(dǎo)型合成酶規(guī)格：48T/96T

腎上腺皮質(zhì)發(fā)育異常同源蛋白抗體規(guī)格 大鼠雌激素(E)ELISA試劑盒 ，英文名： E ELISA Kit

Mouse collagen type IV (Col IV) ELISA Kit 小鼠Ⅳ型膠原(Col Ⅳ)ELISA試劑盒

ELISA 小鼠CXC趨化因子配體16(mouse CXCL16) 48T/96T 進(jìn)口分裝

CLIAKitforLFA-3/CD58ELISAKit大鼠淋巴細(xì)胞功能相關(guān)抗原3規(guī)格：48T/96T

通用型HPV7(HUMANPAPILLOMAVIRUS-7)病毒定量PCR擴(kuò)增檢測(cè)試劑盒20次

ELISAKitET猴子內(nèi)毒素規(guī)格：48T/96T

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 腎上腺皮質(zhì)發(fā)育異常同源蛋白抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）腎上腺皮質(zhì)發(fā)育異常同源蛋白抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。