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產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS10311 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-Cytohesin 1/PSCD1 |
克隆性 | |
靶點(diǎn) | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human PSCD1 |
產(chǎn)品訂購(gòu)信息:
英文名稱 Anti-Cytohesin 1/PSCD1
中文名稱 胞粘蛋白1抗體抗體品牌
別 名 SEC7 and coiled-coil domain-containing protein 1; B2 1; CLM1; CTH 1; CYH1_HUMAN; CYTH1; CYTIP; Cytoadhesin 1; Cytohesin-1; Homolog of secretory protein SEC7; mSec7 1; PH; PH, SEC7 and coiled-coil domain containing protein 1; Pleckstrin homology Sec7 and coiled/coil domains 1; Pleckstrin homology Sec7 and coiled/coil domains protein 1; PSCD1; Sec7; SEC7 homolog A; SEC7 homolog B2 1; SEC7 homolog B2-1; SEC7, yeast, homolog of; Sec7a.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞粘附分子
蛋白分子量 predicted molecular weight: 44kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human PSCD1
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
胞粘蛋白1抗體抗體品牌 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Cytohesin 1 is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. Cytohesin 1 is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. The encoded protein is 83% homologous to that of CYTH2.
Subunit : Interacts with TRIM23 and CYTIP.
Subcellular Location : Cell membrane.
Tissue Specificity : Ubiquitous.
Contains 1 SEC7 domain.
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Anti-HINT1 組氨酸三聚體核苷結(jié)合蛋白1抗體Multi-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh METTL11A 甲基化樣蛋白11抗體(N端) 規(guī)格 0.1ml
CK(Human Creatine Kinase) ELISA Kit 人肌酸激酶 96T
RGS21 英文名稱: G蛋白信號(hào)調(diào)節(jié)蛋白21抗體 0.2ml
C6orf129 英文名稱: 6號(hào)染色體開放閱讀框129抗體 0.2ml
Anti-HINT1 組氨酸三聚體核苷結(jié)合蛋白1抗體Multi-class antibodies規(guī)格: 0.2ml
AMA(Anti-mitochon-drial Anti-body) 人抗線粒體抗體Multi-class antibodies規(guī)格: 48T
Anti-CK19 細(xì)胞角蛋白19抗體Multi-class antibodies規(guī)格: 0.1ml
Rhesus antibody Rh NRSF/REST 神經(jīng)元抑制蛋白抗體 規(guī)格 0.1ml
定影液試劑 定影液試劑 200ml
TSARG7 英文名稱: 形成相關(guān)凋亡蛋白7抗體 0.2ml
C7orf45 英文名稱: 7號(hào)染色體開放閱讀框45抗體 0.2ml
Anti-CK19 細(xì)胞角蛋白19抗體Multi-class antibodies規(guī)格: 0.1ml
Anti-Phospho-RSK2 (Tyr529) /FITC 熒光素標(biāo)記磷酸化核糖體S6激酶RSK2抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-Synaptotagmin-4/FITC 熒光素標(biāo)記突觸結(jié)合蛋白4抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh Agrin 聚集蛋白抗體 規(guī)格 0.1ml
Rabbit Anti-Guinea pig IgG/PE-Cy7 PE-Cy7標(biāo)記的兔抗豚鼠IgG 0.1ml
GLP-1 (7-36) 英文名稱: 胰素樣肽-1抗體 0.1ml
Rhesus antibody Rh Rabbit Anti-Goat IgG/HRP 辣根過氧化物酶標(biāo)記的兔抗羊IgG 規(guī)格 0.1ml
Anti-Synaptotagmin-4/FITC 熒光素標(biāo)記突觸結(jié)合蛋白4抗體IgGMulti-class antibodies規(guī)格: 0.2ml
大鼠可溶性CD40配體(sCD40L)ELISA試劑盒 ,英文名: sCD40L ELISA Kit
人可溶性白細(xì)胞分化抗原40配體(sCD40L)ELISA檢測(cè)試劑盒HumanSolubleClusterofdiffereiation40ligand,sCD40LELISAKit 96T/48T
Sulphadiazine(磺胺嘧啶)免疫試劑盒 Sulphadiazine ELISA Kit
CLIAKitforOFQ/N(MouseorphaninFQ/nociceptin)ELISAKit小鼠孤腓肽規(guī)格:48T/96T
特級(jí)溶菌酶(10毫克/毫升)1毫升
ELISAKitBLI人β內(nèi)酰胺酶抑制劑規(guī)格:48T/96T
大鼠白介素7(IL7)ELISA試劑盒 ,英文名: IL7 ELISA Kit
Human beta galactosidase (beta GAL) ELISA Kit 人β半乳糖苷酶(βGAL)ELISA試劑盒
PlaVitaminD3,VD3ELISAkit 植物維生素D3(VD3)ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforC-1(Humancadherln-relatedneuronalreceptor1)ELISAKit人鈣粘蛋白相關(guān)的神經(jīng)受體1規(guī)格:48T/96T
細(xì)胞游離膽固醇酶連續(xù)循環(huán)熒光定量檢測(cè)試劑盒20次
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Mouse 17 hydroxyprogesterone (17-OHP) ELISA Kit 小鼠17羥孕酮(17-OHP)ELISA試劑盒
ELISA 小鼠N端前腦素(mouse -proBNP) 48T/96T 進(jìn)口分裝
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通用型HCV(HEPATITISVIRUSC)病毒定性檢測(cè)試劑盒20次
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抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 胞粘蛋白1抗體抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)胞粘蛋白1抗體抗體品牌 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。