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產(chǎn)品訂購信息：
英文名稱  Anti-PDZK1 

中文名稱   PDZ結(jié)構(gòu)域PDZK1蛋白抗體說明書 

別    名  CAP70; CFTR associated protein of 70 kDa; CFTR associated protein, 70-KD; CFTR-associated protein of 70 kDa; CLAMP; D3Ertd537e; Dietary Pi-regulated RNA-1; Diphor-1; mPDZK1; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; Na/Pi cotransporter C-terminal-associated protein; NaPi Cap1; NaPi-Cap1; NaPiCap1; NHERF 3; NHERF-3; NHERF3; NHRF3_HUMAN; OTTHUMP00000015572; PDZ domain containing 1; PDZ domain containing protein 1; PDZ domain-containing protein 1; PDZD1; PDZK1; Sodium hydrogen exchanger regulatory factor 3; Sodium-hydrogen exchanger regulatory factor 3; 1700023D20Rik; 2610507N21Rik; 4921513F16Rik; AI267131; AI314638; AL022680; C terminal linking and modulating protein.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Rabbit, Orangutan 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo)

蛋白分子量  predicted molecular weight: 57kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PDZK1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

PDZ結(jié)構(gòu)域PDZK1蛋白抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.

Function : A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function (By similarity).

Subunit : Interacts with PDZK1IP1 and ABCC2. Binds to the C-terminal region of SLC26A3. Interacts via its PDZ1 domain with the C-terminal domain of SCARB1. Forms a heterodimeric complex with SLC9A3R1. Interacts with AKAP2, BCR, CFTR, SLC22A12, SLC22A4, SLC22A5, SLC26A6, SLC9A3R2 and SLC17A1. Component of a complex, composed of PDZK1, SYNGAP1, KLHL17 and NMDA receptors. Interacts (via PDZ1 domain) directly with KLHL17; the interaction is important for integrity of actin cytoskeleton structures in neurons (By similarity).

Subcellular Location : Cytoplasm. Membrane. Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.

Similarity : Belongs to the NHER family.

Contains 4 PDZ (DHR) domains.

Database links :

Entrez Gene: 5174 Human

Entrez Gene: 59020 Mouse

Entrez Gene: 100008900 Rabbit

Entrez Gene: 65144 Rat

Omim: 603831 Human

SwissProt: Q5T2W1 Human

SwissProt: Q9JIL4 Mouse

SwissProt: Q865P3 Rabbit

SwissProt: Q9JJ40 Rat

Unigene: 444751 Human

Unigene: 482226 Mouse

Unigene: 19842 Rat

IL-23(Human Interleukin 23) ELISA Kit 人白介素23Multi-class antibodies規(guī)格： 48T

Anti-CXCL7/NAP-2/PPBP 中性粒細(xì)胞趨化蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Mouse anti-human IgG ascites 小鼠抗人IgG腹水 規(guī)格 1ml

SFT(Human sulfatide) ELISA Kit 人腦苷酯 96T

RelB 英文名稱： 核轉(zhuǎn)錄因子NFKB-RelB蛋白抗體 0.2ml

Cyclin A1 英文名稱： 周期素A1蛋白抗體 0.1ml

Anti-CXCL7/NAP-2/PPBP 中性粒細(xì)胞趨化蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

PR(Progestogerone receptor)Human 人孕激素受體Multi-class antibodies規(guī)格： 48T

Anti-Claudin 4 緊密連接蛋白4抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh NOC3L/FAD24 脂肪細(xì)胞分化因子24 規(guī)格 0.2ml

Human Beta Lactoglobulin, Beta-Lg ELISA Kit 人β乳球蛋白 96T

TUBA1C 英文名稱： 微管相關(guān)蛋白α6抗體 0.1ml

C1orf112 英文名稱： 1號染色體開放閱讀框112抗體 0.2ml

Anti-Claudin 4 緊密連接蛋白4抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-HRG- Beta/FITC 熒光素標(biāo)記抗HRGβ抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-P-HP1/FITC 熒光素標(biāo)記抗異染色質(zhì)蛋白1磷酸化抗體(果蠅)IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ASPP1 p53凋亡刺激蛋白1抗體 規(guī)格 0.1ml

Donkey Anti-Guinea pig IgG/AP 堿性磷酸酶(AP)標(biāo)記的驢抗豚鼠IgG 0.1ml

GLUD2 英文名稱： 谷酸脫氫酶2抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-mouse IgG/PE-Cy5.5 PE-Cy5.5標(biāo)記的兔抗小鼠IgG 規(guī)格 0.1ml

Anti-P-HP1/FITC 熒光素標(biāo)記抗異染色質(zhì)蛋白1磷酸化抗體(果蠅)IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠可溶性血小板內(nèi)皮細(xì)胞粘附分子1(sPECAM-1/sCD31)ELISA試劑盒 ，英文名： sPECAM-1/sCD31 ELISA Kit

人內(nèi)皮細(xì)胞生長因子B(VEGF-B)ELISA檢測試劑盒HumanVascularEndothelialcellGrowthFactorB,VEGF-BELISAKIT 96T/48T

食蟹猴C肽(C-Peptide)免疫試劑盒 Cynomologus Monkey C-Peptide ELISA Kit

CLIAKitforOPGL(MouseOsteoprotegerinLigand)ELISAKIT小鼠骨保護(hù)素配體規(guī)格：48T/96T

糖果糖精(saccharin)含量紫外光譜法定量檢測試劑盒20次

ELISAKitAGPs人阿拉伯半乳糖蛋白規(guī)格：48T/96T

大鼠白細(xì)胞介素25(IL-25)ELISA試劑盒 96T/48T 試劑盒 組裝/原裝

人輪狀病毒(RV)IgM 免疫試劑盒 Human Rotavirus,RV IgM ELISA Kit

MouseαN-acetylglucosaminidase,αNAGELISAKit小鼠αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒規(guī)格：96T/48T

石蠟切片組織EGFR蛋白表達(dá)NBT顯色光學(xué)顯微鏡檢測試劑盒10/20次

HumanProteinZELISA試劑盒人蛋白Z(ProteinZ)ELISA試劑盒規(guī)格：96T/48T

Humaissueinhibitorsofmetalloproteinase4，TIMP-4ELISAKit人基質(zhì)金屬蛋白酶抑制因子4(TIMP-4)ELISA試劑盒規(guī)格：96T/48T

PDZ結(jié)構(gòu)域PDZK1蛋白抗體說明書 大鼠單核細(xì)胞趨化蛋白1(MCP-1)ELISA試劑盒 ，英文名： MCP-1 ELISA Kit

Porcine apolipoprotein B100 (apo-B100) ELISA Kit 豬載脂蛋白B100(apo-B100)ELISA試劑盒

ELISA 小鼠抗激素/加壓素(mouse AVP/ADH) 48T/96T 進(jìn)口分裝

CLIAKitforLPAb-IgA(HumanLegionellaaibodyIgA)ELISAKit人軍團(tuán)菌抗體IgA規(guī)格：48T/96T

通用型DNA克隆試劑盒(不包括內(nèi)切酶)10次

ELISAKitIL-1雞白介素1規(guī)格：48T/96T

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. PDZ結(jié)構(gòu)域PDZK1蛋白抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）PDZ結(jié)構(gòu)域PDZK1蛋白抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。