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產(chǎn)品訂購信息：
英文名稱  Anti-PER2/Period circadian protein 2 

中文名稱   節(jié)律蛋白2抗體規(guī)格 

別    名  Circadian clock protein PERIOD 2; FASPS; hPER 2; hPER2; KIAA0347; PER 2; PER2; Period 2; Period 2 isoform 1; Period circadian protein 2; Period circadian protein homolog 2; Period homolog 2; Period2; FASPS; PER2_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Horse 

產(chǎn)品類型   一抗  

研究領(lǐng)域     免疫學(xué) 染色質(zhì)和核信號(hào) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡

蛋白分子量  predicted molecular weight: 138kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PER2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

節(jié)律蛋白2抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 PER2, a mammalian homologue of the Drosophila period gene, shares a 40% homology with PER1 including the protein dimerization PAS domain. PER2 is a circadian regulator that may act as a transcription factor. It behaves as a negative element in circadian transcriptional loop. PER2 does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hour rhythm in the suprachiasmatic nucleus (SCN) and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina.

Function : Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK NPAS2-BMAL1

Subunit : Homodimer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with PER1 and PER3, and through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts, via its second PAS domain, with TIMELESS in vitro. Interacts with NFIL3 (By similarity). Interacts with PML (By similarity).

Subcellular Location : Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.

Post-translational modifications : Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.

DISEASE : Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.

Similarity : Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 2 PAS (PER-ARNT-SIM) domains.

Database links : UniProtKB/Swiss-Prot: O15055.2

生物鐘基因Per2與, 凋亡有著重要的關(guān)聯(lián)，是機(jī)體維持正常節(jié)律所必需的基因, Per2在。

SLC/CCL21(Human secondary lymphoid-tissue chemokine) ELISA Kit 人二級(jí)淋巴組織趨化因子Multi-class antibodies規(guī)格： 48T

Anti-GTP cyclohydrolase 1/GTP-CH-1 三磷酸鳥苷環(huán)水解酶抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh MMP19 基質(zhì)金屬蛋白酶18/19 規(guī)格 0.2ml

MMP-5(Human matrix metalloproteinase 5) ELISA Kit 人基質(zhì)金屬蛋白酶5 96T

Reelin 英文名稱： 絡(luò)絲蛋白抗體 0.1ml

Claudin 8 英文名稱： 緊密連接蛋白8抗體 0.2ml

Anti-GTP cyclohydrolase 1/GTP-CH-1 三磷酸鳥苷環(huán)水解酶抗體Multi-class antibodies規(guī)格： 0.2ml

Hsp-60(Mouse heat shock protein 60)ELISA Kit 小鼠熱休克蛋白60Multi-class antibodies規(guī)格： 48T

Anti-CLEC13D/MMR1/CD206/FITC 熒光素標(biāo)記巨噬細(xì)胞甘露糖受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh DISC1 (CT) DISC1 C端抗體 規(guī)格 0.2ml

Streptavidin/PE PE標(biāo)記鏈霉親和素 0.1ml

LRRTM2 英文名稱： 富含亮酸重復(fù)跨膜神經(jīng)元蛋白2抗體 0.2ml

新霉素檢測(cè)試劑盒 96孔/盒 動(dòng)物組織/水產(chǎn)/蜂蜜/奶

Anti-CLEC13D/MMR1/CD206/FITC 熒光素標(biāo)記巨噬細(xì)胞甘露糖受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-HSL (Ser660)/FITC 熒光素標(biāo)記磷酸化荷爾蒙敏感脂肪酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-PIBF/FITC 熒光素標(biāo)記孕激素誘導(dǎo)阻斷因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ASM/Acid sphingomyelinase 酸性神經(jīng)鞘磷脂酶抗體 規(guī)格 0.1ml

Donkey Anti-Guinea pig IgG/Cy3 Cy3標(biāo)記的驢抗豚鼠IgG 0.1ml

GLUT6 英文名稱： 葡萄糖轉(zhuǎn)運(yùn)蛋白6抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-mouse IgG/Gold 膠體金標(biāo)記的兔抗小鼠IgG 規(guī)格 0.5ml

Anti-PIBF/FITC 熒光素標(biāo)記孕激素誘導(dǎo)阻斷因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠粒細(xì)胞集落刺激因子(G-CSF)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人神經(jīng)絲蛋白H(NF-H)免疫試劑盒 Human NF-H ELISA Kit

MouseHelicobacterpyloriIgG,Hp-IgGELISAKit小鼠幽門螺旋菌IgG(Hp-IgG)ELISA試劑盒規(guī)格：96T/48T

Androgeeceptor(抗人;抗兔;抗小鼠;抗大鼠)100微克

Humanmembranecofactorprotein,MCPELISA試劑盒人膜輔蛋白(MCP/CD46)ELISA試劑盒規(guī)格：96T/48T

Humanplasminogenactivatorinhibitor1，PAI-1ELISAKit人纖溶酶原激活物抑制因子1(PAI-1)ELISA試劑盒規(guī)格：96T/48T

大鼠半乳凝素3(GAL3)ELISA試劑盒 ，英文名： GAL3 ELISA Kit

Rat gonadoopin releasing hormone (GH) ELISA Kit 大鼠激素釋放激素(GH)ELISA試劑盒

NudeMouseClaracellprotein,CC16ELISAKit 裸鼠克拉拉細(xì)胞蛋白(CC16)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforColIII(HumanCollageypeIII)ELISAKIT人Ⅲ型膠原規(guī)格：48T/96T

細(xì)胞乙?；M蛋白H4染色質(zhì)免疫沉淀分析(CHIP)試劑盒10次

RatCiatesyhase,CSELISAKit大鼠檸檬酸合酶(CS)ELISA試劑盒規(guī)格：96T/48T

節(jié)律蛋白2抗體規(guī)格 大鼠膽固醇(CH)ELISA試劑盒 96T/48T 試劑盒 組裝/原裝

人膜聯(lián)蛋白Ⅵ(ANX-Ⅵ)免疫試劑盒 Human Annexin Ⅵ,ANX-Ⅵ ELISA Kit

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抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 節(jié)律蛋白2抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）節(jié)律蛋白2抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。