|
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10251 |
| 應(yīng)用范圍 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 |
| CAS編號(hào) | |
| 抗體名 | Anti-P14ARF/CDKN2A |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human P16/CDKN2A |
中文名稱 基因p16/p14/P19抗體品牌
英文名稱 Anti-P14ARF/CDKN2A
別 名 CDKN2A; p16INK4a; INK4; P16INK4; ARF; CCM2; CDK4 Inhibitor; CDK4 inhibitor p16 INK4; CDK4I; CDKN2; CDKN2A; Cell cycle negative regulator beta; CMM2; Cyclin dependent kinase 4 inhibitor A; Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4); Cyclin Dependent Kinase Inhibitor 2A; Cyclin dependent kinase inhibitor 2A isoform 4; Cyclin dependent kinase inhibitor 2A isoforms 1/2/3; Cyclin dependent kinase inhibitor p16; INK4A; MLM; MTS1; Multiple tumor suppressor 1; Multiple Tumor Supressor 1; p14; p14(ARF); p14ARF; p16; p16 INK4; p16INK4; p16INK4a; p19; p19(ARF); p19Arf; TP16.
產(chǎn)品屬性：
基因p16/p14/P19抗體品牌 濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué)
蛋白分子量 predicted molecular weight: 15kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human P16/CDKN2A
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
基因p16/p14/P19抗體品牌 產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Function : Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.
Subunit : Does not interact with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6. Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9. Interacts with TBRG1 and COMMD1. Interacts with CDKN2AIP and E4F1.
Subcellular Location : Nucleus, nucleolus. Nucleus, nucleoplasm.
細(xì)胞周期失控是的重要原因。p16是近年來發(fā)現(xiàn)的第一個(gè)直接參與細(xì)胞周期調(diào)控的基因，其表達(dá)產(chǎn)物為p16蛋白.
p16基因是一種重要的基因，在正常細(xì)胞中起負(fù)反饋?zhàn)饔?，?dāng)p16基因突變或丟失時(shí)，細(xì)胞增殖失去控制使細(xì)胞無限制地增殖。
p16主要功能是通過抑制CDK4而阻止細(xì)胞由G1期進(jìn)入S期，使細(xì)胞增殖受到限制。用于各種如、惡黑、的研究。目前的研究細(xì)胞周期依賴激酶抑制p16INK4a蛋白在宮頸上皮內(nèi)(CIN)中作為一個(gè)新標(biāo)記物.
p16INK4a的過表達(dá)與HPV E7區(qū)(早期蛋白即致癌基因編碼區(qū))活性有密切相關(guān)性。
p16/CDKN2基因是新近發(fā)現(xiàn)的抑制基因，已有研究表明該基因在許多出現(xiàn)缺失、突變或重排現(xiàn)象.
實(shí)驗(yàn)流程：
全、新、優(yōu)、品、好四大特點(diǎn)：
基因p16/p14/P19抗體品牌 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。
技術(shù)外包服務(wù):
★基因p16/p14/P19抗體品牌 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
正在熱銷的相關(guān)產(chǎn)品：
Human anti-IVIgG antibody ELISA Kit 人抗IVIgG抗體Multi-class antibodies規(guī)格： 48T
Anti-IL-6 白介素6抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh LRCH1 富含亮氨酸重復(fù)家庭蛋白1抗體 規(guī)格 0.2ml
LPS(Human Lipopolysaccharides) ELISA Kit 人脂多糖/內(nèi)毒素 96T
RCL 英文名稱： c-Myc應(yīng)答蛋白抗體 0.2ml
Classical swine fever virus 英文名稱： 豬瘟病毒抗體 0.2ml
Anti-IL-6 白介素6抗體Multi-class antibodies規(guī)格： 0.1ml
sTNF Alpha-RI(humen) 人可溶性壞死因子-受體1Multi-class antibodies規(guī)格： 48T
Anti-C-Mer/MERTK c-mer原癌基因酪氨酸激酶抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh NPY/Neuropeptide Y 神經(jīng)肽Y抗體 規(guī)格 0.1ml
SP Kit(Broad Spectrum) 廣譜SP免疫組化檢測(cè)試劑盒(用于檢測(cè)兔、大鼠、小鼠和豚鼠來源的一抗) 3ml
TCHP 英文名稱： 蛋白TCHP抗體 0.2ml
C7orf13 英文名稱： 7號(hào)染色體開放閱讀框13抗體 0.2ml
Anti-C-Mer/MERTK c-mer原癌基因酪氨酸激酶抗體Multi-class antibodies規(guī)格： 0.1ml
Anti-Ku-70/FITC 熒光素標(biāo)記DNA修復(fù)酶Ku70抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-Phospho-Smad2 (Ser465/467)/FITC 熒光素標(biāo)記磷酸化細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)分子SMAD2抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh ALDH1L1 乙醛脫氫酶1蛋白家族L1抗體 規(guī)格 0.1ml
Rabbit Anti-human IgG/Alexa Fluor 350 Alexa Fluor 350標(biāo)記的兔抗人IgG 0.1ml
GLYAT 英文名稱： 甘胺酸-N-?；D(zhuǎn)移酶抗體 0.2ml
Rhesus antibody Rh Rabbit Anti-Guinea pig IgG/Gold 膠體金標(biāo)記的兔抗豚鼠IgG 規(guī)格 0.5ml
Anti-Phospho-Smad2 (Ser465/467)/FITC 熒光素標(biāo)記磷酸化細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)分子SMAD2抗體IgGMulti-class antibodies規(guī)格： 0.2ml
大鼠磷蛋白關(guān)聯(lián)鞘糖脂微區(qū)1(PAG1)ELISA試劑盒 ，英文名： PAG1 ELISA Kit
人生成素受體Tie2(ANG-R-Tie2)ELISA檢測(cè)試劑盒Humanangiopoietieceptoie2,ANG-R-Tie2ELISAkit 96T/48T
新德里超級(jí)細(xì)菌金屬β-內(nèi)酰胺酶-1(NDM-1)免疫試劑盒 New Delhi metallo-beta-lactamase-1,NDM-1 ELISA kit
CLIAKitforOT/BGP(HumanOsteocalcin/Boneglaprotein)ELISAkit人骨鈣素/骨谷氨酸蛋白規(guī)格：48T/96T
糖蛋白膠回收試劑盒20次
ELISAKitIL-13人白介素13規(guī)格：48T/96T
大鼠苯酚磺基轉(zhuǎn)移酶(PST)ELISA試劑盒 ，英文名： PST ELISA Kit
Rat follicle stimulating hormone (FSH) ELISA Kit 大鼠促卵泡素(FSH)ELISA試劑盒
Ratalcoholdehydrogenase,ADHELISAKit 大鼠乙醇脫輕酶(ADH)ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforCollagenaseI(MouseCollagenaseTypeI)ELISAKit小鼠膠原酶I規(guī)格：48T/96T
細(xì)胞乙酰膽堿脂酶活性染色試劑盒50次
RatClusterin,CLUELISAKit大鼠凝聚素(CLU)ELISA試劑盒規(guī)格：96T/48T
基因p16/p14/P19抗體品牌 大鼠膽囊收縮素八肽(CCK8)ELISA試劑盒 ，英文名： CCK8 ELISA Kit
Human PL12 aibody / ai alanyl NA syhetase (PL12/AlaRS 人PL12抗體/抗氨酰NA合成酶(PL12/AlaRS
Ratthrombomodulin,TMELISAKit 大鼠調(diào)節(jié)蛋白(TM)ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforc(Humancardiacisoformofopni)ELISAKit人心肌特異性肌鈣蛋白T規(guī)格：48T/96T
細(xì)胞蘇木素染色試劑盒50次
RatEpithelial-Cadherin,E-CadELISAKit大鼠E鈣黏蛋白/上皮鈣黏蛋白(E-Cad)ELISA試劑盒規(guī)格：96T/48T
|
|
|
|
