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脂滴包被蛋白Perilipin-A抗體規(guī)格 英文名稱  Anti-Perilipin A

中文名稱  脂滴包被蛋白Perilipin-A抗體規(guī)格 

別    名 Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin; Perilipin-1; Perilipin 1; PerilipinA; PLIN; PLIN1; PLIN1_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep

產(chǎn)品類型   一抗  

研究領(lǐng)域    心 免疫學(xué) 合成與降解 病

蛋白分子量  predicted molecular weight: 57kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Perilipin A

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

脂滴包被蛋白Perilipin-A抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids.

Function : Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.

Subunit : Interacts with ABHD5.

Subcellular Location : Lipid droplet. Note=Lipid droplet surface-associated.

Post-translational modifications : Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.

DISEASE : Defects in PLIN1 are the cause of familial partial lipodystrophy type 4 (FPLD4) [MIM:613877]. FPLD4 is a form of lipodystrophy characterized by loss of subcutaneous adipose tissue primarily affecting the lower limbs, insulin-resistant diabetes mellitus, hypertriglyceridemia, and hypertension.

Similarity : Belongs to the perilipin family.

Database links : UniProtKB/Swiss-Prot: O60240.2

脂肪細(xì)胞中貯存的甘油三酯的水解是人和動(dòng)物體內(nèi)精細(xì)過(guò)程，與維持機(jī)體能量的動(dòng)態(tài)平衡和代謝正常密切相關(guān)。Perilipin A是成熟脂肪細(xì)胞中脂滴膜上表達(dá)量較多的一種結(jié)構(gòu)和功能蛋白，是多條脂解通路的終末靶點(diǎn)之一，對(duì)脂肪細(xì)胞的脂肪分解起著關(guān)鍵的調(diào)控作用.

perilipin可能在脂肪分解調(diào)控中起到“分子開關(guān)”的作用。蛋白激酶A（PKA）、細(xì)胞外信號(hào)調(diào)節(jié)激酶（ERK）等信號(hào)轉(zhuǎn)導(dǎo)通路參與了脂肪分解。僅（TNFα）、過(guò)氧化物酶體增殖物激活受體γ（PPAγ）激動(dòng)劑、瘦素（leptin）均可以影響perilipin的表達(dá)。近年來(lái)研究，perilipin可通過(guò)蛋白酶體途徑來(lái)調(diào)節(jié)其蛋白量的表達(dá)。脂肪分解調(diào)控中的關(guān)鍵蛋白perilipin對(duì)、、等多種代謝性疾病及相關(guān)聯(lián)。

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