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產品訂購信息：
英文名稱  Anti-p53BP1/53BP1 

中文名稱   p53結合蛋白1抗體品牌 

別    名  53 BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53BP1; TP53 BP1; TP53BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; TP53B_HUMAN; tumor protein 53-binding protein, 1.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Dog, Rabbit 

產品類型   一抗  

研究領域     免疫學

蛋白分子量  predicted molecular weight: 213kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human p53BP1/53BP1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

p53結合蛋白1抗體品牌 產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品介紹 p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.

Function : May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.

Subunit : Interacts with IFI202A (By similarity). Binds to the central domain of p53/TP53. May form homooligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20' (H4K20me2). Has low affinity for histone H4 containing monomethylated 'Lys-20' (H4K20me1). Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20' (H4K20me3). Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3. Interacts with MUM1/EXPAND1. Interacts with CHEK2; modulates CHEK2 phosphorylation at 'Thr-68' in response to infrared. Interacts with MSL1; this interaction may be required for MSL1 DNA repair activity, but not for histone acetyltransferase activity.

Subcellular Location : Nucleus. Chromosome; centromere; kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.

Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.

DISEASE : Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.

Similarity : Contains 2 BRCT domains.

Database links : UniProtKB/Swiss-Prot: Q12888.2

p53結合蛋白1(53BP1)是細胞周期檢查點激酶蛋白，是新近發(fā)現的DNA損傷修復通路中兩個非常重要的蛋白激酶MDC1和53BP1蛋白之一,目前多用于方面的研究。

bFGF-4(Human basic fibroblast growth factor 4) ELISA Kit 人堿性成纖維細胞生長因子4Multi-class antibodies規(guī)格： 48T

Anti-GRP94/gp96 葡萄糖調節(jié)蛋白94抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh MMP-7 基質金屬蛋白酶-7抗體 規(guī)格 0.1ml

MMP-8(Human Matrix metalloproteinase 8/Neutrophil collagenase) ELISA kit 人基質金屬蛋白酶8/中性粒細胞膠原酶 96T

RASA1 英文名稱： Rho GTP酶激活蛋白1/骨肥大綜合征相關蛋白抗體 0.2ml

CRELD2 英文名稱： 富含半胱酸與表皮生長因子樣蛋白2抗體 0.2ml

Anti-GRP94/gp96 葡萄糖調節(jié)蛋白94抗體Multi-class antibodies規(guī)格： 0.1ml

Substance P 人P物質Multi-class antibodies規(guī)格： 48T

Anti-Connexin 43 Cx-43蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh NHLRC1 肌痙攣相關EPM2蛋白抗體 規(guī)格 0.2ml

Human Attacin,Att ELISA Kit 人抗菌肽 96T

TNFAIP2 英文名稱： 壞死因子誘導蛋白2(TNFα-IP 2)抗體 0.2ml

Connexin 31.1 英文名稱： 間隙連接蛋白31.1抗體 0.1ml

Anti-Connexin 43 Cx-43蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-MAFbx/Fbx32/Atrogin-1/FITC 熒光素標記泛素蛋白連接酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-TEM 1/CD248/FITC 熒光素標記內涎蛋白/內皮唾液酸蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ADAMTS7(NT) 整合素樣金屬蛋白酶與凝血酶1型-7抗體 (N端抗體) 規(guī)格 0.1ml

Rabbit Anti-chicken IgM/FITC FITC標記的兔抗雞IgM 0.3ml

GNAT3 英文名稱： G蛋白轉錄因子α3抗體 0.2ml

Rhesus antibody Rh Rabbit anti-chicken IgG whole serum 兔抗雞IgG抗血清 規(guī)格 1ml

Anti-TEM 1/CD248/FITC 熒光素標記內涎蛋白/內皮唾液酸蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠免疫球蛋白E(IgE)ELISA試劑盒 ，英文名： IgE ELISA Kit

人內皮抑素(ES)ELISA檢測試劑盒HumanEndostatin,ESELISAKit 96T/48T

鴨生長激素1(GH1)免疫試劑盒 Duck growth hormone growth hormone 1,GH1 ELISA kit

CLIAKitforPACAP(Humanpituitaryadenylatecyclaseactivatingpolypeptide)ELISAKit人垂體腺苷酸環(huán)化酶激活肽規(guī)格：48T/96T

水中腸球菌(Eerococci)基因熒光定量檢測試劑盒20次

ELISAKitLCA/CD45人白細胞共同抗原規(guī)格：48T/96T

大鼠不對稱二甲基精酸(ADMA)ELISA試劑盒 ，英文名： ADMA ELISA Kit

Mouse p53 (p53) ELISA Kit 小鼠p53(p53)ELISA試劑盒

ELISA 小鼠多巴胺(mouse Dopamine) 48T/96T 進口分裝

CLIAKitforLBP(Humanlipolysaccharidebindingprotein)ELISAKit人脂多糖結合蛋白規(guī)格：48T/96T

通用型vero毒素大腸桿菌志賀氏毒素-2(VTEC-Shigatoxin2)試劑盒20次

ELISAKitLv/Vn紅螯光殼螯蝦卵黃脂磷蛋白規(guī)格：48T/96T

p53結合蛋白1抗體品牌 大鼠低氧誘導因子1α(HIF-1α)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人內皮型合成酶(eNOS)免疫試劑盒 Human Endothelial niic oxide syhase,eNOS ELISA Kit

MousesubstancePreceptor,SP-RELISAKit小鼠P物質受體(SP-R)ELISA試劑盒規(guī)格：96T/48T

冰凍切片組織線粒體復合物I蛋白表達NBT顯色光學顯微鏡檢測試劑盒10/20次

HumanplateletmembraneglycoproteinⅣ,GP-ⅣELISA試劑盒人血小板膜糖蛋白Ⅳ(GP-Ⅳ)ELISA試劑盒規(guī)格：96T/48T

HumanSomelysin-1,ST1ELISAKit人基質裂解素(ST1)ELISA試劑盒規(guī)格：96T/48T

抗體的生物素化標記實驗要點：

1. p53結合蛋白1抗體品牌 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）p53結合蛋白1抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。