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產(chǎn)品訂購(gòu)信息：
英文名稱(chēng)  Anti-phospho-RAD9(Ser277) 

中文名稱(chēng)   磷酸化細(xì)胞周期檢查控制蛋白質(zhì)抗體品牌 

別    名  RAD9A(phospho S277); Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9A_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源   Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Mouse 

產(chǎn)品類(lèi)型   一抗 磷酸化抗體  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 細(xì)胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 43kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from mouse RAD9 around the phosphorylation site of Ser277 [LC(p-S)PK] 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化細(xì)胞周期檢查控制蛋白質(zhì)抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq].

Function : Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Subunit : Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.

Subcellular Location : Nucleus.

Similarity : Belongs to the rad9 family.

Database links : UniProtKB/Swiss-Prot: Q99638.1 human

UniProtKB/Swiss-Prot: Q9Z0F6.1 mouse

Rad9由391個(gè)AA構(gòu)成，Rad9有很多重要生物學(xué)功能，如：可調(diào)控DNA損傷反應(yīng)、調(diào)節(jié)細(xì)胞的周期、對(duì)損傷的DNA進(jìn)行修復(fù)、誘導(dǎo)細(xì)胞凋亡、調(diào)控部分基因的轉(zhuǎn)錄、具有3’端到5’端核酸外切酵素(exonuclease)的活性、促進(jìn)核糖核酸的生合成以及參與胚胎發(fā)育過(guò)程。曾有報(bào)道指出Rad9在調(diào)節(jié)細(xì)胞周期、誘導(dǎo)細(xì)胞凋亡及維持整個(gè)基因體的完整性等方面的生物功能都和p53極為相似，因此Rad9及p53這兩種蛋白在功能上可能具有相互協(xié)調(diào)作用。

CYFRA21-1(Human cytokeratin fragment antigen 21-1) ELISA Kit 人細(xì)胞角蛋白21-1片段Multi-class antibodies規(guī)格： 48T

Anti-Phospho-HER3 (Tyr1222) 磷酸化HER3受體抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh MIA2 抑制活性蛋白2抗體 規(guī)格 0.2ml

uPA/PLAU(Human urokinase-type plasminogen activator) ELISA Kit 人尿激酶型纖溶酶原激活物 96T

RNF149 英文名稱(chēng)： 環(huán)指蛋白149抗體 0.2ml

CEP72 英文名稱(chēng)： 中心體蛋白72抗體 0.1ml

Anti-Phospho-HER3 (Tyr1222) 磷酸化HER3受體抗體Multi-class antibodies規(guī)格： 0.1ml

Leptin 瘦素(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-CD2 CD2抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh PAI-2/SerpinB2 纖溶酶原激活物抑制因子2抗體(血漿酶原激活酶抑制因子-2) 規(guī)格 0.1ml

COQ7 Kit Human 人 COQ7 ELISA配對(duì)抗體 ELISA

Tsg101 英文名稱(chēng)： 易感基因101蛋白抗體 0.1ml

C6orf150 英文名稱(chēng)： 6號(hào)染色體開(kāi)放閱讀框150抗體 0.1ml

Anti-CD2 CD2抗體Multi-class antibodies規(guī)格： 0.2ml

FAS/CD95 ELISA Kit 大鼠凋亡相關(guān)因子Multi-class antibodies規(guī)格： 48T

Anti-APPL1/FITC 熒光素標(biāo)記抗銜接因子蛋白含pH域磷酸酪氨酸結(jié)合域和亮氨酸拉鏈基元1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FAM40A FAM家族蛋白40A抗體 規(guī)格 0.2ml

MECF(ouse eosinophil chemotactic factor) ELISA Kit 小鼠嗜酸粒細(xì)胞趨化因子 96T

MIA2 英文名稱(chēng)： 抑制活性蛋白2抗體 0.2ml

ALDH1B1 英文名稱(chēng)： 乙醛脫氫酶5抗體 0.1ml

Anti-APPL1/FITC 熒光素標(biāo)記抗銜接因子蛋白含pH域磷酸酪氨酸結(jié)合域和亮氨酸拉鏈基元1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠骨髓間充質(zhì)干細(xì)胞(BMSCs)

CHL 中國(guó)倉(cāng)鼠肺細(xì)胞

SERPINA6 Others Mouse 小鼠 SerpinA6 / CBG 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0054Calu-3(人)5×106cells/瓶×2

WST-1細(xì)胞活力和增殖檢測(cè)試劑盒WST

EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞(拉祜族);KM9406 人內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

CL-0096HCT 116(人細(xì)胞)5×106cells/瓶×2

SPG21 Others Human 人 SPG21 桿狀病毒-昆蟲(chóng)細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人神經(jīng)束膜細(xì)胞裂解物HPCL

TT細(xì)胞，人骨髓樣細(xì)胞 人腎小球系膜細(xì)胞,HMC細(xì)胞 神經(jīng)小膠質(zhì)細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

兔腎細(xì)胞;RK13

SLIK1 Others Human 人 SLIK1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

磷酸化細(xì)胞周期檢查控制蛋白質(zhì)抗體品牌 大鼠骨髓間充質(zhì)干細(xì)胞(BMSCs)

CHL 中國(guó)倉(cāng)鼠肺細(xì)胞

SERPINA6 Others Mouse 小鼠 SerpinA6 / CBG 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0054Calu-3(人)5×106cells/瓶×2

WST-1細(xì)胞活力和增殖檢測(cè)試劑盒WST

EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞(拉祜族);KM9406 人內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酸化細(xì)胞周期檢查控制蛋白質(zhì)抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）磷酸化細(xì)胞周期檢查控制蛋白質(zhì)抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。