|
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10184 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 |
| CAS編號(hào) | |
| 抗體名 | Anti-PJA2/RNF131 |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human PJA2/RNF131 |
環(huán)指蛋白131抗體說明書 英文名稱 Anti-PJA2/RNF131
中文名稱 環(huán)指蛋白131抗體說明書
別 名 E3 ubiquitin protein ligase Praja2; E3 ubiquitin-protein ligase Praja-2; KIAA0438; Neurodap 1; Neurodap1; PJA 2; PJA2; PJA2_HUMAN; Praja 2; Praja 2 RING H2 motif containing; praja ring finger 2; Praja ring finger 2 E3 ubiquitin protein ligase; Praja2; RING finger protein 131; RNF 131; RNF131.
產(chǎn)品屬性：
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Cow
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué)
蛋白分子量 predicted molecular weight: 78kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human PJA2/RNF131
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
環(huán)指蛋白131抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 PJA2, also known as E3 ubiquitin-protein ligase praja-2, RNF131 (ring finger protein 131) or Neurodap1, is a 708 amino acid protein that contains one ring-type zinc finger and exists as two alternatively spliced isoforms. Significantly conserved in chimpanzee, dog, cow, mouse, rat, chicken and zebrafish, PJA2 shares 52% identity with PJA1, which is involved in protein ubiquitination in brain and may play a role in X-linked mental retardation. Encoded by a gene that maps to human chromosome 5q21.3, PJA2 localizes to both endoplasmic reticulum and Golgi apparatus membranes. Participating in E2-dependent, E3 ubiquitin-protein ligase activity, PJA2 binds to a variety of E2s and interacts with ubiquitin-conjugating enzymes, such as UBE2D2, in vitro.
Function : Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes.
Subunit : Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. The phosphorylated form interacts with PRKAR1A, PRKAR2A and PRKAR2B. Binds the catalytic subunits of cAMP-dependent protein kinase. [SUBCELLULAR LOCATION] Cytoplasm. Cell membrane. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Cell junction, synapse (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Note=Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses (By similarity). Co-localizes with PRKAR2A and PRKAR2B in the cytoplasm and the cell membrane.
Similarity : Contains 1 RING-type zinc finger.
實(shí)驗(yàn)流程：
全、新、優(yōu)、品、好四大特點(diǎn)：
環(huán)指蛋白131抗體說明書 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。
正在熱銷的相關(guān)產(chǎn)品：
TAP(Human trypsinogen activation peptide) ELISA Kit 人胰蛋白酶原激活肽Multi-class antibodies規(guī)格： 48T
Anti-IAA (Indole-3-Acetic Acid) 吲哚乙酸抗體/植物生長(zhǎng)素抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh MAPKAP Kinase 2 原活化蛋白激酶活化的蛋白激酶2抗體 規(guī)格 0.2ml
UGCG(Human UDP-glucose ceramide glucosyltransferase) ELISA Kit 人尿苷二磷酸葡萄糖神經(jīng)酰胺葡萄糖基轉(zhuǎn)移酶 96T
ROM1 英文名稱： 視網(wǎng)膜感光細(xì)胞外節(jié)膜蛋白1抗體 0.2ml
CRIPTO 英文名稱： 衍化生長(zhǎng)因子抗體(N端) 0.2ml
Anti-IAA (Indole-3-Acetic Acid) 吲哚乙酸抗體/植物生長(zhǎng)素抗體Multi-class antibodies規(guī)格： 0.2ml
TRBF1 (Telomeric repeat binding factor 1) 端粒體復(fù)制結(jié)合因子1抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-CaMK2 beta 鈣/鈣調(diào)素依賴蛋白激酶2b抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh PDE5A 磷酸二酯酶5A抗體 規(guī)格 0.2ml
Actin,Pan 濃縮液 0.1ml 進(jìn)口分裝
TRPV3 英文名稱： 辣椒素受體3抗體 0.1ml
C1orf229 英文名稱： 1號(hào)染色體開放閱讀框229抗體 0.2ml
Anti-CaMK2 beta 鈣/鈣調(diào)素依賴蛋白激酶2b抗體Multi-class antibodies規(guī)格： 0.1ml
NADPH peptide 還原型輔酶Ⅱ抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-AQP4 水通道蛋白-4抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh phospho-ATG4D(Ser341) 磷酸化自噬相關(guān)蛋白4D抗體 規(guī)格 0.1ml
山羊抗大鼠 IgG(H+L)/HRP 0.1ml 美國(guó)Jackson公司分裝
VGLL3 英文名稱： 轉(zhuǎn)錄輔助因子退變樣蛋白3抗體 0.2ml
DIRAS1 英文名稱： RAS相關(guān)抑細(xì)胞生長(zhǎng)蛋白1抗體 0.2ml
Anti-AQP4 水通道蛋白-4抗體Multi-class antibodies規(guī)格： 0.1ml
細(xì)胞名稱
BHK-21 [C-13] 倉鼠腎成纖維細(xì)胞
IFNGR2 Others Mouse 小鼠 IFNGR2 人細(xì)胞裂解液 (陽性對(duì)照)
CL-0142LM/TK(小鼠胸腺激酶缺陷細(xì)胞)5×106cells/瓶×2
人腎皮質(zhì)上皮細(xì)胞裂解物HRCEpiCL
SV40轉(zhuǎn)染人成骨細(xì)胞;hFOB 1.19 人心房肌細(xì)胞完全培養(yǎng)基 100mL
CL-0119HuH-6(人肝母細(xì)胞瘤細(xì)胞)5×106cells/瓶×2
IgG3 Others Mouse 小鼠 IgG3-Fc 人細(xì)胞裂解液 (陽性對(duì)照)
小鼠肝內(nèi)膽管上皮細(xì)胞完全培養(yǎng)基 100mL
MC3T3-E1 Subclone 14小鼠顱頂前骨細(xì)胞亞克隆14 MC3T3-E1 Subclone 14 in mouse parietal bone cell sub clone 14 before a-MEM培養(yǎng)基+10%FBS
OSM Protein Mouse 重組小鼠 Oncostatin M / OSM 蛋白 (His 標(biāo)簽)
RS1(大鼠皮膚成纖維樣細(xì)胞) 5×106cells/瓶×2 THP-1(人單核細(xì)胞)
環(huán)指蛋白131抗體說明書 細(xì)胞名稱
BHK-21 [C-13] 倉鼠腎成纖維細(xì)胞
IFNGR2 Others Mouse 小鼠 IFNGR2 人細(xì)胞裂解液 (陽性對(duì)照)
CL-0142LM/TK(小鼠胸腺激酶缺陷細(xì)胞)5×106cells/瓶×2
人腎皮質(zhì)上皮細(xì)胞裂解物HRCEpiCL
SV40轉(zhuǎn)染人成骨細(xì)胞;hFOB 1.19 人心房肌細(xì)胞完全培養(yǎng)基 100mL
技術(shù)外包服務(wù):
★環(huán)指蛋白131抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
|
|
|
|
