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產(chǎn)品訂購信息：
英文名稱  Anti-PACRG 

中文名稱   麻瘋病和共同調(diào)節(jié)蛋白抗體品牌 

別    名  GLUP; Molecular chaperone/chaperonin binding protein; PARK 2 co regulated; PARK 2 coregulated; PARK2 co regulated; PARK2 coregulated; PARK2CRG; Parkin co regulated gene protein; Parkin Co-regulated Gene; Parkin coregulated gene protein; RP3 495O10.2; PACRG_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Horse, Rabbit, Sheep, Zebrafish 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細菌及 Alzheimer's

蛋白分子量  predicted molecular weight: 33kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PACRG 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

麻瘋病和共同調(diào)節(jié)蛋白抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The deduced 257 amino acid protein PACRG (Parkin co-regulated gene) shows potential links to the ubiquitin/proteasome system. PACRG and Parkin are attached in a head-to-head arrangement on opposite DNA strands and share a common 5' flanking promoter region. The PACRG gene maps to chromosome 6q26; Northern blot analysis detects PACRG expression in all tissues examined except placenta. Using a positional cloning strategy in 197 Vietnamese leprosy simplex families (i.e. families with two unaffected parents and one affected child), significant connections between leprosy and 17 markers in the 5' regulatory region that PARK2 and PACRG share were observed. Possession of two or more of the 17 risk alleles is highly predictive of leprosy. PACRG is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes.

Function : PACRG is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes.

Subunit : Forms a large molecular chaperone complex containing heat shock proteins 70 and 90 and chaperonin components. Interacts with STIP1, PARK2, GPR37, HSPA8, TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A, CCT7, CCT8. Interacts with MEIG1 (By similarity).

Tissue Specificity : Expressed in all immune tissues, spleen, lymph nodes, thymus, tonsils, leukocyte and bone marrow. Expressed also in heart, brain, skeletal muscle, kidney, lung and pancreas. Expressed in primary Schwann cells and very weakly by monocyte-derived macrophages the primary host cells of Mycobacterium leprae, the causative agent of leprosy. Component of Lewy bodies, intraneuronal inclusions found in the brain of Parkinson disease patients.

CTAP III (Human connective tissue-activating peptide III ,CTAP III ) ELISA Kit 人結(jié)締組織活化肽ⅢMulti-class antibodies規(guī)格： 48T

Anti-FOXF1 叉頭蛋白F1抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Mouse Anti-rabbit IgG/AP 堿性磷酸酶(AP)標記的小鼠抗兔IgG 規(guī)格 0.1ml

UFC(Human urinary free cortisol) ELISA Kit 人尿游離皮質(zhì)醇 96T

SP-A 英文名稱： 肺表面活性蛋白A抗體 0.1ml

CCDC94 英文名稱： 卷曲螺旋結(jié)構(gòu)域蛋白94抗體 0.2ml

Anti-FOXF1 叉頭蛋白F1抗體Multi-class antibodies規(guī)格： 0.2ml

PKB (human) 人蛋白激酶BMulti-class antibodies規(guī)格： 48T

Anti-CXCR1/IL-8RA 細胞表面趨化因子受體1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh NGX6 細胞相關(guān)基因6抗體 規(guī)格 0.1ml

Human retinoic acid inducible gene/RIG-like receptor,RLR-9 ELISA Kit 人視黃酸誘導(dǎo)基因/RIG-1樣受體 96T

TRPV2 英文名稱： 辣椒素受體樣蛋白1抗體 0.1ml

CHMP2A 英文名稱： 腺癌標志物CHMP2A抗體 0.1ml

Anti-CXCR1/IL-8RA 細胞表面趨化因子受體1抗體Multi-class antibodies規(guī)格： 0.1ml

1,3- BetaD-Glu ELISA Kit 大鼠1,3-βD葡葡糖苷酶Multi-class antibodies規(guī)格： 48T

Anti-AQP4/FITC 熒光素標記水通道蛋白-4蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FAM21C FAM21C蛋白抗體 規(guī)格 0.2ml

MIF(Human Macrophage Migration Inhibitory Factor) ELISA Kit 人巨噬細胞移動抑制因子 96T

Mkl1 英文名稱： myocardin相關(guān)轉(zhuǎn)錄因子抗體 0.2ml

ASAH1 英文名稱： 酸性神經(jīng)酰胺酶1抗體 0.2ml

Anti-AQP4/FITC 熒光素標記水通道蛋白-4蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

MN-c, 小鼠皮質(zhì)神經(jīng)元

BHK-21 敘利亞倉鼠腎細胞

DLL1 Others Rat 大鼠 DLL1 / Delta-like 人細胞裂解液 (陽性對照)

CL-0150MDA-MB-231(人癌細胞)5×106cells/瓶×2

人平滑肌細胞裂解物HPSMCL

腺病毒轉(zhuǎn)化的人胚腎細胞;AAV-293 人腎足突細胞完全培養(yǎng)基 100mL

CL-0120HuH-7(人細胞)5×106cells/瓶×2

MAPKAPK3 Others Human 人 MAPKAPK3 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

人牙周膜成纖維細胞裂解物HPLFL

SW 1088[SW-1088;SW1088]細胞，人腦星型細胞 人細胞,OS-RC-2細胞 氣管上皮細胞Many types of cells包裝：5 × 105次方(1ml)

兔晶體上皮細胞永生系;N/N1003A(RLE)

MSR1 Others Human 人 MSR1 / SCARA1 / CD204 人細胞裂解液 (陽性對照)

麻瘋病和共同調(diào)節(jié)蛋白抗體品牌 MN-c, 小鼠皮質(zhì)神經(jīng)元

BHK-21 敘利亞倉鼠腎細胞

DLL1 Others Rat 大鼠 DLL1 / Delta-like 人細胞裂解液 (陽性對照)

CL-0150MDA-MB-231(人癌細胞)5×106cells/瓶×2

人平滑肌細胞裂解物HPSMCL

腺病毒轉(zhuǎn)化的人胚腎細胞;AAV-293 人腎足突細胞完全培養(yǎng)基 100mL

抗體的生物素化標記實驗要點：

1. 麻瘋病和共同調(diào)節(jié)蛋白抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

抗體的鑒定：

1）麻瘋病和共同調(diào)節(jié)蛋白抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。