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| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10181 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-PHD2 |
| 克隆性 | |
| 靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
| 形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
| 宿主 | 詳見(jiàn)說(shuō)明書(shū) |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human PHD2 |
中文名稱(chēng) 脯氨酸羥化酶2抗體費(fèi)用
英文名稱(chēng) Anti-PHD2
別 名 SM 20; Zinc finger MYND domain containing protein 6; C1ORF12; Chromosome 1 Open Reading Frame 12; ECYT 3; ECYT3; EGL 9 homolog of C. elegans 1; EGL nine (C.elegans) homolog 1; Egl nine homolog 1; Egl nine like protein 1; EGLN 1; EGLN1; HIF PH2; HIF Prolyl Hydroxylase 2; HIFP4H 2; HIFPH2; HPH 2; HPH2; Hypoxia inducible factor prolyl hydroxylase 2; ORF13; P4H2; PHD 2; PHD2; PNAS 118; PNAS 137; Prolyl Hydroxylase Domain Containing Protein 2; Rat Homolog of SM20; SM20; ZMYND6.
產(chǎn)品屬性：
脯氨酸羥化酶2抗體費(fèi)用 濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類(lèi)型 polyclonal
交叉反應(yīng) Human, Mouse, Pig, Cow, Rabbit
產(chǎn)品類(lèi)型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細(xì)胞凋亡 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 47kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human PHD2
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
脯氨酸羥化酶2抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 PHD2 protein catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. In the presence of oxygen, PHD2 converts specific prolyl residues in HIF alpha to hydroxyproline, leading to HIF alpha proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF levels are stable. This results in increased angiogenesis as HIF1 alpha regulates the expression of many angiogenesis-related genes.
Subcellular Location : Cytoplasm. Nucleus.
Tissue Specificity : According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle.
Similarity : Contains 1 Fe2OG dioxygenase domain.
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技術(shù)外包服務(wù):
★脯氨酸羥化酶2抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
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