|
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10100 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-OAT-3 |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from rat OAT-3 N-terminus |
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 陰離子轉(zhuǎn)運(yùn)蛋白-3抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
產(chǎn)品訂購(gòu)信息：
英文名稱 Anti-OAT-3
中文名稱 陰離子轉(zhuǎn)運(yùn)蛋白-3抗體說明書
別 名 Organic anion transporter 3; hOAT3; OAT3; SLC22A8; Solute carrier family 22 member 8; S22A8_RAT.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg 1ml/1mg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)運(yùn)蛋白
蛋白分子量 predicted molecular weight: 59kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from rat OAT-3 N-terminus
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
陰離子轉(zhuǎn)運(yùn)蛋白-3抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Human organic anion transporter (OAT) 3 (SLC22A8) is localized to the basolateral membranes of renal tubular epithelial cells and plays a critical role in the excretion of anionic compounds. Recent advances in molecular biology have identified three organic anion transporter families: the organic anion transporter (OAT) family encoded by SLC22A, the organic anion transporting peptide (OATP) family encoded by SLC21A (SLCO), and the multidrug resistance-associated protein (MRP) family encoded by ABCC. These families play critical roles in the transepithelial transport of organic anions in the kidneys as well as in other tissues such as the liver and brain. Among these families, the OAT family plays the central role in renal organic anion transport. Knowledge of these three families at the molecular level, such as substrate selectivity, tissue distribution, and gene localization, is rapidly increasing.
Function : Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).
Subcellular Location : Basolateral cell membrane; Multi-pass membrane protein (Potential). Note=Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity).
Tissue Specificity : Expressed in kidney.
Similarity : Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
Database links : UniProtKB/Swiss-Prot: Q9R1U7.1
OAT3陰離子轉(zhuǎn)運(yùn)蛋白是一類分布廣泛的膜蛋白，通過介導(dǎo)Cl－／HCO－3跨膜轉(zhuǎn)運(yùn)參與細(xì)胞內(nèi)pH、細(xì)胞體積及細(xì)胞內(nèi)氯離子濃度的調(diào)節(jié)。
抗體的鑒定：
1）陰離子轉(zhuǎn)運(yùn)蛋白-3抗體說明書 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
IL-23 人白介素-23Multi-class antibodies規(guī)格： 48T
Anti-ChRM2 毒蕈堿型乙酰膽堿受體M2抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh NUCKS1 核酪蛋白激酶和細(xì)胞周期蛋白依賴性激酶底物1抗體 規(guī)格 0.2ml
TRIquickLS 液體樣品RNA提取試劑 20ml
UNC80 英文名稱： UNC80蛋白抗體 0.2ml
CPT2 英文名稱： 肉毒堿棕櫚?；D(zhuǎn)移酶2抗體 0.2ml
Anti-ChRM2 毒蕈堿型乙酰膽堿受體M2抗體Multi-class antibodies規(guī)格： 0.1ml
IL-15 人白介素-15Multi-class antibodies規(guī)格： 48T
Anti-Tetracycline 四環(huán)素抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh Nucleostemin 核干細(xì)胞因子抗體(鳥嘌呤核苷酸結(jié)合蛋白3) 規(guī)格 0.2ml
FITC 異硫氫酸熒光素 50mg
TPST1 英文名稱： 酪蛋白轉(zhuǎn)移酶1抗體 0.2ml
CDKN2D 英文名稱： 細(xì)胞周期蛋白依賴性激酶抑制劑2D抗體 0.2ml
Anti-Tetracycline 四環(huán)素抗體Multi-class antibodies規(guī)格： 0.2ml
PCX/PODXL(Podocalyxin) 足細(xì)胞特異蛋白抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-ATP4B 氫ATP酶通道蛋白抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh phospho-ANAPC1(Ser377) 磷酸化細(xì)胞周期末期促進(jìn)復(fù)合蛋白APC1抗體 規(guī)格 0.1ml
P53 濃縮液 0.1ml 進(jìn)口分裝
UBXD2 英文名稱： UBX結(jié)構(gòu)域域蛋白D2抗體 0.2ml
DRIP5 英文名稱： 多巴胺受體相互作用蛋白5抗體 0.2ml
Anti-ATP4B 氫ATP酶通道蛋白抗體Multi-class antibodies規(guī)格： 0.1ml
MIF Protein Mouse 重組小鼠 MIF / Migration Inhibitory Factor 蛋白
MDA-MB-231 人癌細(xì)胞
IGSF11 Others Human 人 IGSF11 / BTIGSF 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
SK-BR-3人腺癌細(xì)胞 SK-BR-3 human breast adenocarcinoma cells DMEM培養(yǎng)基+10%FBS
小鼠紅細(xì)胞;MEL
人胚胎氣管組織來源細(xì)胞;CCC-HBE-2 人肺泡上皮細(xì)胞完全培養(yǎng)基 100mL
CL-0288MA-891(小鼠癌高轉(zhuǎn)移細(xì)胞)5×106cells/瓶×2
CLEC4F Others Rat 大鼠 CLEC4F / CLECSF13 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人前脂肪細(xì)胞-內(nèi)臟cDNAHPA-v cDNA
BCaP-37細(xì)胞，人癌細(xì)胞 猴腎細(xì)胞系,MA-104細(xì)胞 腸內(nèi)皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
TE-13(人細(xì)胞) 5×106cells/瓶×2
HBdMEC Pellet 人膀胱微內(nèi)皮細(xì)胞團(tuán)塊 > 1 mio.cells 胰酶/EDTA消化液T/E
陰離子轉(zhuǎn)運(yùn)蛋白-3抗體說明書 MIF Protein Mouse 重組小鼠 MIF / Migration Inhibitory Factor 蛋白
MDA-MB-231 人癌細(xì)胞
IGSF11 Others Human 人 IGSF11 / BTIGSF 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
SK-BR-3人腺癌細(xì)胞 SK-BR-3 human breast adenocarcinoma cells DMEM培養(yǎng)基+10%FBS
小鼠紅細(xì)胞;MEL
人胚胎氣管組織來源細(xì)胞;CCC-HBE-2 人肺泡上皮細(xì)胞完全培養(yǎng)基 100mL
|
|
|
|
