|
| 產地 | 進口、國產 |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS10076 |
| 應用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-Orexin B |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human Orexin B |
產品訂購信息：
英文名稱 Anti-Orexin B
中文名稱 增食欲素B/欲激素B說明書
別 名 HCRT; Hcrt2; Orexin 2; Orexin-2; hypocretin (orexin) neuropeptide precursor; Hypocretin 2; hypocretin; NRCLP1; Orexin; Orexin precursor; OX; PPOX; prepro orexin; OREX_HUMAN; PPORX.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Human, Dog, Pig, Cow, Rabbit, Sheep
產品類型 一抗
研究領域 細胞生物 免疫學 神經生物學
蛋白分子量 predicted molecular weight: 3kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Orexin B
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
增食欲素B/欲激素B說明書 產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 The hypothalamus acts as a major regulatory center involved in the control of feeding behavior and energy homeostasis. Several neuropeptides and proteins have been shown to be involved in the regulation of these processes. Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B(hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro-orexin, which is encoded by a gene localized to human chromosome 17q21. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behavior.
Function : Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
Subcellular Location : Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.
Post-translational modifications : Specific enzymatic cleavages at paired basic residues yield the different active peptides.
DISEASE : Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1) [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Note=Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients.
Similarity : Belongs to the orexin family.
Database links : UniProtKB/Swiss-Prot: O43612.1
Anti-Caspase-6 (NT)/FITC 熒光素標記半胱胺酸蛋白酶蛋白-6抗體(N端)IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-HA tag/HRP 辣根過氧化物酶標記HA tag標簽抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh CG6856/CG6856-PA 果蠅CG6856-PA抗體 規(guī)格 0.5ml
TRAF2/TNF-R2 (TNF receptor-associated factor 2) 壞死因子受體相關因子2抗原 0.5ml
IL-4 英文名稱： 白介素4抗體 0.1ml
Rhesus antibody Rh TLR9/CD289 Toll樣受體9抗體 規(guī)格 0.1ml
Anti-HA tag/HRP 辣根過氧化物酶標記HA tag標簽抗體IgGMulti-class antibodies規(guī)格： 0.2ml
human PL-A2 (phospholipase A2) 人磷脂酶A2Multi-class antibodies規(guī)格： 48T
Anti-ChRM3 毒蕈堿型乙酰膽堿受體M3抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh NUBP2 核苷酸結合蛋白2抗體 規(guī)格 0.2ml
TRIquick 總RNA提取試劑 20ml
Tomosyn/STXBP5 英文名稱： 突觸融合蛋白結合蛋白5抗體 0.2ml
CNOT2 英文名稱： 細胞表面趨化因子受體4相關蛋白2抗體 0.2ml
Anti-ChRM3 毒蕈堿型乙酰膽堿受體M3抗體Multi-class antibodies規(guī)格： 0.1ml
phospho-AKT1/PKB1/2/3(pThr473)peptide 磷酸化蛋白激酶B抗原(絲氨酸磷酸化位點：473)Multi-class antibodies規(guī)格： 0.5mg
Anti-Aurora B 有絲分裂激酶B抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-AKT/PKB(Ser473) 磷酸化蛋白激酶B抗體 規(guī)格 0.1ml
Mesothelin(1:60) 間皮瘤 濃縮液 0.1ml 進口分裝
UNC5C 英文名稱： 神經突起生長誘導因子受體UNC5B抗體 0.2ml
DYNC1I1 英文名稱： 胞漿動力蛋白中間鏈1抗體 0.1ml
Anti-Aurora B 有絲分裂激酶B抗體Multi-class antibodies規(guī)格： 0.2ml
HGF Protein Cynomolgus 重組食蟹猴 HGF / Hepatocyte Growth Factor 蛋白
KG-1 人髓性紅細胞
ULBP2 Others Human 人 ULBP2 / N2DL-2 人細胞裂解液 (陽性對照)
HeLa 229人細胞 HeLa 229 human cervical cancer cells RPMI-1640(GIBCO)+10%FBS
中國倉鼠肺細胞;R 1610 [R1610]
小鼠周神經成纖維細胞(MPNF)(5×105) HL-60, 人早幼粒細胞 Human
CL-0336FC33(人胚胎腎細胞(Asp-2基因修飾))5×106cells/瓶×2
CS Others Human 人 Ciate syhase / CS 桿狀病毒-昆蟲細胞裂解液 (陽性對照)
人腸平滑肌細胞總RNAHISMC NA
山羊皮膚細胞;WGS1 大鼠心肌細胞，H9c2細胞 Ca759細胞，615小鼠株
人胚胎成纖維樣細胞;HEH2
HA Others H1N2 甲型 H1N2 (A/swine/Guangxi/13/2006) 血凝素HA1 (Hemagglutinin) 人細胞裂解液 (陽性對照)
增食欲素B/欲激素B說明書 HGF Protein Cynomolgus 重組食蟹猴 HGF / Hepatocyte Growth Factor 蛋白
KG-1 人髓性紅細胞
ULBP2 Others Human 人 ULBP2 / N2DL-2 人細胞裂解液 (陽性對照)
HeLa 229人細胞 HeLa 229 human cervical cancer cells RPMI-1640(GIBCO)+10%FBS
中國倉鼠肺細胞;R 1610 [R1610]
小鼠周神經成纖維細胞(MPNF)(5×105) HL-60, 人早幼粒細胞 Human
抗體的生物素化標記實驗要點：
1. 增食欲素B/欲激素B說明書 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；
7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產生競爭作用；
8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。
抗體的鑒定：
1）增食欲素B/欲激素B說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
