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產(chǎn)品訂購信息：
英文名稱  Anti-OTX1 + OTX2 

中文名稱   轉(zhuǎn)錄因子OTX1+OTX2抗體規(guī)格 

別    名  FLJ38361; Homeobox protein OTX 1; Homeobox protein OTX1; Homeobox protein OTX2; MCOPS 5; MCOPS5; MGC15736; MGC45000; Orthodenticle 1; Orthodenticle 2; Orthodenticle homeobox 1; Orthodenticle homeobox 2; Orthodenticle homolog 1; Orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Orthodenticle1; Orthodenticle2; Otx 1; Otx 2; otx1; OTX1_HUMAN; otx2; OTX2_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 37/32kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human OTX1 + OTX2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

轉(zhuǎn)錄因子OTX1+OTX2抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Transcription factors, OTX1 and OTX2, are two murine homologs of the Drosophila orthodenticle (OTD), show a limited amino acid sequence divergence. OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, specification and regionalization of the brain. OTX1 is involved in corticogenesis, sensory organ development and pituitary functions, while OTX2 is necessary earlier in development, for the correct anterior neural plate specification and organization of the primitive streak. OTX2 is also required in the early specification of the neuroectoderm, which is destined to become the fore-midbrain, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism. A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning. The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, respectively.

Function : Function: Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Subcellular Location : Nucleus

Tissue Specificity : Expressed in brain.

DISEASE : Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.

Similarity : Belongs to the paired homeobox family. Bicoid subfamily. Contains 1 homeobox DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: P32242.1 UniProtKB/Swiss-Prot: P32243.1

sEPCR ELISA Kit 大鼠可溶性內(nèi)皮細(xì)胞蛋白C受體Multi-class antibodies規(guī)格： 48T

Anti-AR(phospho Ser213/Ser791)/FITC 熒光素標(biāo)記磷酸化雄激素受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FAM156A/TMEM2 跨膜蛋白29抗體 規(guī)格 0.2ml

TPO-Ab ELISA Kit 大鼠抗甲狀腺過氧化物酶抗體 96T

Myf6 英文名稱： 生肌調(diào)節(jié)因子6抗體 0.2ml

phospho-ACTH (Ser168) 英文名稱： 磷酸化促腎上腺皮質(zhì)激素ACTH抗體 0.1ml

Anti-AR(phospho Ser213/Ser791)/FITC 熒光素標(biāo)記磷酸化雄激素受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

STM 人可溶性調(diào)節(jié)蛋白Multi-class antibodies規(guī)格： 48T

Anti-Phospho-DARPP32 (Thr75) 磷酸化多巴胺/cAMP調(diào)節(jié)磷蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Neurocan 神經(jīng)粘蛋白抗體 規(guī)格 0.1ml

human Prostaglandin D2-methoxime,PGD2-MOX ELISA KIT 人甲肟素D2 96T

TNFRSF12A 英文名稱： 壞死因子配體超家族成員12A抗體 0.1ml

CXCL5 英文名稱： 上皮中性粒細(xì)胞活化肽78抗體 0.1ml

Anti-Phospho-DARPP32 (Thr75) 磷酸化多巴胺/cAMP調(diào)節(jié)磷蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Adipo R2(adiponectin receptor 2) 脂聯(lián)素受體-2(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-Bad 相關(guān)死亡促進(jìn)因子Bad抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Phospho-Ack1(Tyr284) 磷酸化Ack1抗體 規(guī)格 0.1ml

LRP/MVP 耐藥蛋白 濃縮液 0.1ml 進(jìn)口分裝

ULBP3 英文名稱： UL16結(jié)合蛋白3抗體 0.2ml

CHST3 英文名稱： 碳水化合物磺基轉(zhuǎn)移酶3抗體 0.1ml

Anti-Bad 相關(guān)死亡促進(jìn)因子Bad抗體Multi-class antibodies規(guī)格： 0.1ml

FIGF Protein Rat 重組大鼠 VEGF-D / VEGFD / FIGF 蛋白 (Fc 標(biāo)簽)

HO-8910 人細(xì)胞

KDR Others Human 人 VEGFR2 / Flk-1 / CD309 / KDR 人細(xì)胞裂解液 (陽性對照)

ZR-75-30人癌細(xì)胞 Human breast cancer cell line ZR-75-30 RPMI-1640(GIBCO)+10%FBS

非洲綠猴腎細(xì)胞;VERO C1008 (E6)

大鼠星形膠質(zhì)細(xì)胞(RA)(5×105) Caki-1, 人腎透明細(xì)胞癌細(xì)胞 Human

CL-0381MDA-MB-175VII(人導(dǎo)管癌細(xì)胞)5×106cells/瓶×2

LIPF Others Human 人 Gasic lipase / LIPF 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

人心肌細(xì)胞-心室總RNAHCF-av NA

大耳山羊腎細(xì)胞;LDG-2 皮下結(jié)締組織細(xì)胞，A9細(xì)胞 CT26.WT[CT26WT]細(xì)胞，小鼠細(xì)胞

人胚肺二倍體細(xì)胞;KMB-17

HA Others H11N2 甲型 H11N2 (A/duck/Yangzhou/906/2002) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽性對照)

轉(zhuǎn)錄因子OTX1+OTX2抗體規(guī)格 FIGF Protein Rat 重組大鼠 VEGF-D / VEGFD / FIGF 蛋白 (Fc 標(biāo)簽)

HO-8910 人細(xì)胞

KDR Others Human 人 VEGFR2 / Flk-1 / CD309 / KDR 人細(xì)胞裂解液 (陽性對照)

ZR-75-30人癌細(xì)胞 Human breast cancer cell line ZR-75-30 RPMI-1640(GIBCO)+10%FBS

非洲綠猴腎細(xì)胞;VERO C1008 (E6)

大鼠星形膠質(zhì)細(xì)胞(RA)(5×105) Caki-1, 人腎透明細(xì)胞癌細(xì)胞 Human

抗體的生物素化標(biāo)記實驗要點(diǎn)：

1. 轉(zhuǎn)錄因子OTX1+OTX2抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）轉(zhuǎn)錄因子OTX1+OTX2抗體規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。