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| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS10053 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-OTX2 |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human OTX2 (15-105aa) |
產(chǎn)品訂購信息：
英文名稱 Anti-OTX2
中文名稱 轉(zhuǎn)錄因子OTX2抗體費(fèi)用
別 名 CPHD6; Homeobox protein OTX2; MCOPS 5; MCOPS5; MGC45000; Orthodenticle 2; Orthodenticle homeobox 2; Orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Orthodenticle2; Otx 2; otx2; OTX2_HUMAN.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 環(huán)指蛋白
蛋白分子量 predicted molecular weight: 32kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human OTX2 (15-105aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
轉(zhuǎn)錄因子OTX2抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Transcription factors, OTX1 and OTX2, are two murine homologs of the Drosophila orthodenticle (OTD), show a limited amino acid sequence divergence. OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, specification and regionalization of the brain. OTX1 is involved in corticogenesis, sensory organ development and pituitary functions, while OTX2 is necessary earlier in development, for the correct anterior neural plate specification and organization of the primitive streak. OTX2 is also required in the early specification of the neuroectoderm, which is destined to become the fore-midbrain, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism. A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning. The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, respectively.
Function : Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Subcellular Location : Nucleus.
Tissue Specificity : Expressed in brain.
DISEASE : Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.
Similarity : Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain.
Database links : UniProtKB/Swiss-Prot: P32243.1
Anti-APEX1/Ref-1 /FITC 熒光素標(biāo)記氧化還原因子1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-Phospho-PLC gamma 1 (Ser1248)/FITC 熒光素標(biāo)記磷酸化磷酯酶Cγ1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh COMMD10 銅代謝結(jié)構(gòu)域蛋白10抗體 規(guī)格 0.2ml
TPO(Thyroid Peroxidase) 甲狀腺過氧化物酶(抗原) 0.5mg
JHD1 英文名稱： JmjC結(jié)構(gòu)域組蛋白去甲基化酶H3-K36抗體 0.1ml
Rhesus antibody Rh Tuberin/TSC2 馬鈴薯球蛋白(性硬化)抗體 規(guī)格 0.2ml
Anti-Phospho-PLC gamma 1 (Ser1248)/FITC 熒光素標(biāo)記磷酸化磷酯酶Cγ1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Survivin 細(xì)胞凋亡抑制因子的一種(抗原)Multi-class antibodies規(guī)格： 0.5mg
Anti-CD82/KAI1 轉(zhuǎn)移抑制蛋白1抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh OVCA1 相關(guān)基因1抗體 規(guī)格 0.2ml
CFH Kit Human 人 Complement Factor H / CFH ELISA配對抗體 ELISA
TNFR1 英文名稱： 壞死因子受體1抗體 0.1ml
Phospho-CRMP2 (Thr514+Ser518) 英文名稱： 磷酸化二氫嘧啶酶樣2抗體 0.1ml
Anti-CD82/KAI1 轉(zhuǎn)移抑制蛋白1抗體Multi-class antibodies規(guī)格： 0.1ml
IL-2 ELISA KIT 大鼠白介素2Multi-class antibodies規(guī)格： 48T
Anti-BAD(phospho Ser75,Ser99,Ser118) /FITC 熒光素標(biāo)記磷酸化相關(guān)死亡促進(jìn)因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh ETHE1 乙基腦病蛋白抗體 規(guī)格 0.2ml
HSP47 ELISA Kit 大鼠熱休克蛋白47 96T
MYPT1 英文名稱： 肌球蛋酸酶抗體 0.1ml
Amylin 英文名稱： 相關(guān)肽/胰島淀粉樣肽抗體 0.1ml
Anti-BAD(phospho Ser75,Ser99,Ser118) /FITC 熒光素標(biāo)記磷酸化相關(guān)死亡促進(jìn)因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml
PGF Protein Mouse 重組小鼠 PIGF / PLGF 蛋白
HL-60 人原髓細(xì)胞細(xì)胞
F9 Others Mouse 小鼠 Coagulation Factor IX / FIX / F9 人細(xì)胞裂解液 (陽性對照)
COLO 320DM 人結(jié)直腸腺癌細(xì)胞 COLO 320DM human colorectal adenocarcinoma cells RPMI-1640(GIBCO)+10%FBS
恒河猴胚腎細(xì)胞;FRhK-4 [FRhK4]
大鼠周神經(jīng)成纖維細(xì)胞(RPNF)(5×105) HEC-1A, 人細(xì)胞系 Human
CL-0383MDA-MB-436(人癌細(xì)胞)5×106cells/瓶×2
CXADR Others Mouse 小鼠 CXADR / CAR 人細(xì)胞裂解液 (陽性對照)
人角膜上皮細(xì)胞總RNAHCEpiC NA
ZR75-1細(xì)胞，人癌細(xì)胞 129小鼠ES細(xì)胞,D3細(xì)胞 WM35, 人細(xì)胞
MV3(人細(xì)胞) 5×106cells/瓶×2
HUAEC Pellet 人臍動脈內(nèi)皮細(xì)胞團(tuán)塊 > 1 mio.cells 神經(jīng)細(xì)胞培養(yǎng)基NM-prf
轉(zhuǎn)錄因子OTX2抗體費(fèi)用 PGF Protein Mouse 重組小鼠 PIGF / PLGF 蛋白
HL-60 人原髓細(xì)胞細(xì)胞
F9 Others Mouse 小鼠 Coagulation Factor IX / FIX / F9 人細(xì)胞裂解液 (陽性對照)
COLO 320DM 人結(jié)直腸腺癌細(xì)胞 COLO 320DM human colorectal adenocarcinoma cells RPMI-1640(GIBCO)+10%FBS
恒河猴胚腎細(xì)胞;FRhK-4 [FRhK4]
大鼠周神經(jīng)成纖維細(xì)胞(RPNF)(5×105) HEC-1A, 人細(xì)胞系 Human
抗體的生物素化標(biāo)記實驗要點(diǎn)：
1. 轉(zhuǎn)錄因子OTX2抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）轉(zhuǎn)錄因子OTX2抗體費(fèi)用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
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