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產(chǎn)品訂購(gòu)信息：
英文名稱(chēng)  Anti-OST-beta 

中文名稱(chēng)   有機(jī)溶質(zhì)轉(zhuǎn)運(yùn)蛋白OSTβ抗體費(fèi)用 

別    名  organic solute transporter beta (Ostbeta); Organic solute transporter subunit beta; Ostbeta; Ost beta; OST-beta; OSTB; OSTB_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat 

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 通道蛋白

蛋白分子量  predicted molecular weight: 14kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human OST-beta 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

有機(jī)溶質(zhì)轉(zhuǎn)運(yùn)蛋白OSTβ抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The heteromeric transporter OST Alpha/OST Beta facilitates the transport of bile and other steroid solutes across the basolateral epithelial cell membrane of intestine, liver, testis, kidney and adrenal gland. OST Alpha/OST Beta expression is induced by bile acids through ligand-dependent transactivation of their genes by FXR (Farnesoid X-activated receptor). This genetic regulation suggests that in response to changes in intracellular bile acid levels, bile acids adjust the rate of their own efflux from enterocytes. OST Beta is a 128 amino acid single-pass transmembrane protein that requires OST Alpha to localize to the plasma membrane. Coexpression of OST Alpha and OST Beta is also required to convert the OST Alpha subunit to a mature glycosylated endoglycosidase H-resistant form, suggesting that co-expression facilitates trafficking of OST Alpha through the golgi apparatus. Though widely expressed, OST Beta is present at highest levels in ileum.

Function : Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids. Modulates SLC51A glycosylation, membrane trafficking and stability activities.

Subunit : Interacts with SLC51A. The Ost-alpha/Ost-beta complex is a heterodimer composed of alpha (SLC51A) and beta (SLC51B) subunit; induces the transport of SLC51A from the reticulum endoplasmic to the plasma membrane.

Subcellular Location : Cell membrane. Mainly restricted to the lateral and basal membranes of ileal enterocytes.

Tissue Specificity : Widely expressed with a high expression in ileum. Expressed in testis, colon, liver, small intestine, kidney, ovary and adrenal gland; and at low levels in heart, lung, brain, pituitary, thyroid gland, uterus, prostate, mammary gland and fat.

Similarity : Belongs to the OST-beta family.

Database links : UniProtKB/Swiss-Prot: Q86UW2.2

TCC C5b-9 ELISA Kit 大鼠末端補(bǔ)體復(fù)合物C5b-9Multi-class antibodies規(guī)格： 48T

Anti-Phospho-Tyrosine Hydroxylase (Ser31) 磷酸化酪氨酸羥化酶抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh GDF3 生長(zhǎng)分化因子3抗體 規(guī)格 0.1ml

TNFsR- II (Mouse Tumor necrosis factor soluble receptor II ) ELISA KIT 小鼠壞死因子可溶性受體Ⅱ 96T

NARG2 英文名稱(chēng)： 谷酸受體調(diào)節(jié)蛋白2抗體 0.2ml

ANKHD1 英文名稱(chēng)： 病毒制約錨定蛋白抗體 0.2ml

Anti-Phospho-Tyrosine Hydroxylase (Ser31) 磷酸化酪氨酸羥化酶抗體Multi-class antibodies規(guī)格： 0.1ml

Visfatin-2(CT)hu、mo、rat、MK 內(nèi)脂素/內(nèi)臟脂肪素(抗人、大、小鼠、猴：N端多肽)前B細(xì)胞克隆增強(qiáng)因子、內(nèi)肥素、腹脂素Multi-class antibodies規(guī)格： 0.5mg

Anti-CD59 CD59抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh P2Y1R/P2ry1 P2Y1受體抗體/嘌呤受體抗體 規(guī)格 0.1ml

RRM2B Kit Human 人 RRM2B / P53R2 ELISA配對(duì)抗體 ELISA

TMEM59 英文名稱(chēng)： 跨膜蛋白59抗體 0.2ml

COMMD1 英文名稱(chēng)： 銅代謝結(jié)構(gòu)域蛋白1抗體 0.2ml

Anti-CD59 CD59抗體Multi-class antibodies規(guī)格： 0.2ml

RASSF1A(Ras association domain family 1A) Ras相關(guān)區(qū)域家族1A抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Bax Bax抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho C-Met/HGFR(Tyr1365) 磷酸化原癌基因c-Met抗體 規(guī)格 0.1ml

Keratin13 濃縮液 0.1ml 進(jìn)口分裝

UFC1 英文名稱(chēng)： 泛素結(jié)合酶UFC1抗體 0.2ml

Chp2 英文名稱(chēng)： 鈣調(diào)磷酸酶B相關(guān)蛋白2抗體 0.1ml

Anti-Bax Bax抗體Multi-class antibodies規(guī)格： 0.1ml

TNFSF11 Protein Mouse 重組小鼠 RANKL / OPGL / TNFSF11 / CD254 蛋白 (Fc 標(biāo)簽)

HAL-01 人急性混合型細(xì)胞

CD302 Others Mouse 小鼠 CD302 / CLEC13A 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

HCT-8 [HRT-18]人回盲細(xì)胞 HCT-8 [HRT-18] human ileocecal carcinoma cells RPMI-1640(GIBCO)+10%FBS

SV40T轉(zhuǎn)化的人胚腎細(xì)胞;293T

人肺間充質(zhì)干細(xì)胞(肺)(HPMSC)(5×105) U251人細(xì)胞 Human

CL-0408NEC(人細(xì)胞)5×106cells/瓶×2

ANGPTL4 Others Human 人 ANGPTL4 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人脊髓星形膠質(zhì)細(xì)胞RNAHA-sp miRNA5 μg

MCF-7(OLD)細(xì)胞，人癌細(xì)胞 小鼠細(xì)胞,RenCa細(xì)胞 HL-02 [L-02,HL-7702], 正常人肝上皮細(xì)胞

MDA-MB-468(人癌細(xì)胞) 5×106cells/瓶×2

HUVEC Growth Medium 2single donor Pellet #N/A > 1 mio.cells 軟骨細(xì)胞培養(yǎng)基CM

有機(jī)溶質(zhì)轉(zhuǎn)運(yùn)蛋白OSTβ抗體費(fèi)用 TNFSF11 Protein Mouse 重組小鼠 RANKL / OPGL / TNFSF11 / CD254 蛋白 (Fc 標(biāo)簽)

HAL-01 人急性混合型細(xì)胞

CD302 Others Mouse 小鼠 CD302 / CLEC13A 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

HCT-8 [HRT-18]人回盲細(xì)胞 HCT-8 [HRT-18] human ileocecal carcinoma cells RPMI-1640(GIBCO)+10%FBS

SV40T轉(zhuǎn)化的人胚腎細(xì)胞;293T

人肺間充質(zhì)干細(xì)胞(肺)(HPMSC)(5×105) U251人細(xì)胞 Human

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 有機(jī)溶質(zhì)轉(zhuǎn)運(yùn)蛋白OSTβ抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）有機(jī)溶質(zhì)轉(zhuǎn)運(yùn)蛋白OSTβ抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。