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磷酸化核有絲分裂器NuMA蛋白抗體說(shuō)明書 英文名稱  Anti-Phospho-NuMA (Ser395)

中文名稱  磷酸化核有絲分裂器NuMA蛋白抗體說(shuō)明書 

別    名 NUMA 1; NuMA protein; NUMA1; SP H antigen; Structural nuclear protein; Centrophilin stabilizes mitotic spindle in mitotic cells; Nuclear mitotic apparatus protein 1; Nuclear mitotic apparatus protein.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào) 神經(jīng)生物學(xué)

蛋白分子量  predicted molecular weight: 238kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human NuMA around the phosphorylation site of Ser395

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化核有絲分裂器NuMA蛋白抗體說(shuō)明書 產(chǎn)品應(yīng)用   ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis.

Function : Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume. Required for maintenance and establishment of the mitotic spindle poles, functionning as a tether linking bulk microtubules of the spindle to centrosomes. May be involved in coordination of the alignment of the mitotic spindle to the cellular polarity axis, which is a prerequisite for asymmetric cell divisions.Subunit : Homodimer. Also forms multiarm oligomers by association of C-terminal tail domains, oligomers may further assemble to form a hexagonal nuclear lattice-like network. Interacts with TNKS.Subcellular Location : Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton, spindle pole. Note=Resides in the nuclear matrix during interphases. Dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via dynein/dynamin association, it remain there until the anaphase onset. Before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.Isoform Numa-m: Cytoplasm. Note=Mainly clustered at the centrosomal region.Isoform Numa-s: Cytoplasm. Note=Mainly clustered at the centrosomal region.Post-translational modifications : ADP-ribosylated by TNKS during mitosis.Phosphorylated in the C-terminal tail during mitosis, probably by CDK1. Phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.Database links : UniProtKB/Swiss-Prot: Q14980.2NuMA (Nuclear mitotic apparatus protein)是細(xì)胞核基質(zhì)中的一種高分子量蛋白質(zhì)，具有一個(gè)特殊的細(xì)胞周期依賴性.核有絲分裂器蛋白NuMA與人類細(xì)胞的惡性增殖相關(guān).

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