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產(chǎn)地 | 進(jìn)口、國產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號 | CS9943 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500 |
CAS編號 | |
抗體名 | Anti-NR2A/NMDAR2A |
克隆性 | |
靶點 | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標(biāo)識物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human NR2A/NMDAR2A(860-900/1464aa) |
產(chǎn)品訂購信息:
英文名稱 Anti-NR2A/NMDAR2A
中文名稱 谷氨酸受體2A抗體品牌
別 名 Glutamate [NMDA] receptor subunit epsilon 1; Glutamate [NMDA] receptor subunit epsilon-1; Glutamate receptor; Glutamate receptor ionotropic N methyl D aspartate 2A; GRIN 2A; GRIN2A; HNR2A; N methyl D aspartate receptor channel, subunit epsilon 1; N Methyl D Aspartate Receptor Subtype 2A; N methyl D aspartate receptor subunit 2A; N-methyl D-aspartate receptor subtype 2A; NMDA receptor subtype 2A; NMDA Receptor Type 2A; NMDAR 2A; NMDAR2A; NMDE1_HUMAN; NR 2A; NR2A; OTTHUMP00000160135; OTTHUMP00000174531.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細(xì)胞膜受體
蛋白分子量 predicted molecular weight: 163kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human NR2A/NMDAR2A(860-900/1464aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
谷氨酸受體2A抗體品牌 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 NMDA (N-methyl-D-aspartate) receptors are a class of ionotropic glutamate receptors. NMDA channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D).
Function : NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits (By similarity).Subunit : Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4. Interacts with HIP1 and NETO1 (By similarity). Interacts with LRFN2 (By similarity).Subcellular Location : Cell membrane; Multi-pass membrane protein (By similarity). Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein (By similarity).Similarity : Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.Database links : UniProtKB/Swiss-Prot: Q12879.1
TXB2(Human Thromboxane B2) ELISA Kit 人素B2Multi-class antibodies規(guī)格: 48T
Anti-lamin C 核纖層蛋白C抗體Multi-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh KLHL14 Kelch樣蛋白14抗體 規(guī)格 0.2ml
Inhbb(Human inhibin beta-B) ELISA Kit 人抑制素β-B 96T
Protein S 英文名稱: 蛋白S抗體 0.2ml
CDCA7L 英文名稱: 細(xì)胞分裂周期相關(guān)蛋白JPO2抗體 0.2ml
Anti-lamin C 核纖層蛋白C抗體Multi-class antibodies規(guī)格: 0.2ml
bFGF-9 ELISA Kit 大鼠堿性成纖維細(xì)胞生長因子9Multi-class antibodies規(guī)格: 48T
Anti-ApoH/FITC 熒光素標(biāo)記載脂蛋白H抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh FAM50B FAM50B蛋白抗體 規(guī)格 0.2ml
IL-17(Human Interleukin 17) ELISA Kit 人白介素17 96T
MRPS22 英文名稱: 線粒體核糖體蛋白S22抗體 0.1ml
Aspartate Aminotransferase 英文名稱: 谷草轉(zhuǎn)酶抗體 0.2ml
Anti-ApoH/FITC 熒光素標(biāo)記載脂蛋白H抗體IgGMulti-class antibodies規(guī)格: 0.2ml
ACA(Mouse anti-centrol and centrosome antibody)ELISA Kit 小鼠抗中性粒/中心體抗體Multi-class antibodies規(guī)格: 48T
Anti-ocludin-5/FITC 熒光素標(biāo)記緊密連接蛋白-5抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh DLL3 Notch信號通路Delta樣配體3抗體 規(guī)格 0.2ml
BSA/HRP 辣根過氧化物酶標(biāo)記血清白蛋白 0.1ml
LC3A/B 英文名稱: 自噬微管相關(guān)蛋白輕鏈3A/3B抗體 0.1ml
氯丙嗪檢測試劑盒 96孔/盒 動物組織/水產(chǎn)/蜂蜜/奶
Anti-ocludin-5/FITC 熒光素標(biāo)記緊密連接蛋白-5抗體IgGMulti-class antibodies規(guī)格: 0.2ml
0-玉米瓊脂培養(yǎng)基250g用于白色念珠菌產(chǎn)芽管試驗。
KF鏈球菌瓊脂 100(g) incubation media KF鏈球菌瓊脂 100(g)
印度墨水 Indian ink 100毫升 炭疽桿菌檢測用配套試劑
營養(yǎng)瓊脂(NA)250細(xì)菌計數(shù)(GB標(biāo)準(zhǔn)),不含糖,可作血瓊脂基礎(chǔ),傳代用.也可用于消毒效果測定incubationmedia營養(yǎng)瓊脂(NA)250細(xì)菌計數(shù)(GB標(biāo)準(zhǔn)),不含糖,可作血瓊脂基礎(chǔ),傳代用.也可用于消毒效果測定
Cellobiose-polymyxinEAgar
PotatosucroseMedium
結(jié)晶紫中性紅膽鹽MUG瓊脂(VRBA-MUG) 100(g) incubation media 結(jié)晶紫中性紅膽鹽MUG瓊脂(VRBA-MUG) 100(g)
大腸菌群顯色培養(yǎng)基配套平皿 Coliform Chromogenic Medium Dish 9㎝/塊
改良番茄汁培養(yǎng)基250incubationmedia改良番茄汁培養(yǎng)基250
Voges-Proskauer(V-P)reagentbox
谷氨酸受體2A抗體品牌 甘酸培養(yǎng)基 250(g) incubation media 甘酸培養(yǎng)基 250(g)
脒多粘菌素B瓊脂基礎(chǔ) 250(g) incubation media 脒多粘菌素B瓊脂基礎(chǔ) 250(g)
碳酸氫瓊脂基礎(chǔ) 250(g) incubation media 碳酸氫瓊脂基礎(chǔ) 250(g)
指示選擇性培養(yǎng)基基礎(chǔ) 250(g) incubation media 指示選擇性培養(yǎng)基基礎(chǔ) 250(g)
抗體的生物素化標(biāo)記實驗要點:
1. 谷氨酸受體2A抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用;
8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)谷氨酸受體2A抗體品牌 抗體的效價鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。