![]() |
產(chǎn)地 | 進(jìn)口、國產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS9937 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-Nurr1 |
克隆性 | |
靶點(diǎn) | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human Nurr1 N-terminus |
產(chǎn)品訂購信息:
英文名稱 Anti-Nurr1
中文名稱 核受體相關(guān)因子-1抗體費(fèi)用
別 名 Nur-ralated factor 1; Nuclear receptor-related factor 1; Orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; Regenerating liver nuclear receptor 1; RNR-1; SL-322; Nuclear orphan receptor HZF-3; T-cell nuclear receptor NOT;HZF 3; HZF3; Immediate-early response protein NOT; Intermediate early receptor protein; NGFI B/nur77 beta type transcription factor homolog; NOT; NR4A2; NR4A2_HUMAN; Nuclear receptor subfamily 4 group A member 2; Nur related protein 1 homolog; Nurr 1;RNR 1; RNR1; TINUR; Transcriptionally inducible nuclear receptor; Transcriptionally inducible nuclear receptor related; Transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Pig, Cow, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 染色質(zhì)和核信號(hào) 神經(jīng)生物學(xué) 表觀遺傳學(xué)
蛋白分子量 predicted molecular weight: 67kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Nurr1 N-terminus
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
核受體相關(guān)因子-1抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Nuclear receptor and transcription factor; plays a role in development and maintenance of neurons synthesizing the neurotransmitter dopamine This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Four transcript variants encoding four distinct isoforms have been identified for this gene. Additional alternate splice variants may exist, but their full length nature has not been determined.
Function : Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.Subunit : Interacts with SFPQ, NCOR2, SIN3A and HADC1. The interaction with NCOR2 increases in the absence of PITX3 (By similarity).Subcellular Location : Nucleus.Tissue Specificity : Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.Similarity : Belongs to the nuclear hormone receptor family. NR4 subfamily.Contains 1 nuclear receptor DNA-binding domain.Database links : UniProtKB/Swiss-Prot: P43354.1該抗體和類固醇/甲狀腺素受體密切相關(guān)的早期應(yīng)急反應(yīng)基因,也受各種生長因子的誘導(dǎo)。中腦多巴胺神經(jīng)細(xì)胞和腦干細(xì)胞表達(dá)。
Lptn/LTN/XCL1 ELISA Kit 大鼠淋巴細(xì)胞趨化因子Multi-class antibodies規(guī)格: 48T
Anti-Phospho-Tyrosine Hydroxylase (Ser40) 磷酸化酪氨酸羥化酶抗體Multi-class antibodies規(guī)格: 0.1ml
Rhesus antibody Rh GDF8 生長分化因子8/肌肉抑制素抗體 規(guī)格 0.2ml
INH(Human Inhibin) ELISA Kit 人抑制素 96T
NIT1 英文名稱: 水解酶1抗體 0.2ml
Adenylate cyclase 1 英文名稱: 腺苷酸環(huán)化酶1抗體 0.2ml
Anti-Phospho-Tyrosine Hydroxylase (Ser40) 磷酸化酪氨酸羥化酶抗體Multi-class antibodies規(guī)格: 0.1ml
17-KS ELISA Kit 大鼠17-酮類固醇Multi-class antibodies規(guī)格: 48T
Anti-AGER/Gold 金離子標(biāo)記的晚期糖基化終末產(chǎn)物特異性受體抗體Multi-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh FBXO25 F-box蛋白家族FBXO25抗體 規(guī)格 0.2ml
HBeAg(Mouse hepatitis B virus e antigen) ELISA Kit 小鼠e抗原 96T
MRP8 英文名稱: 多藥耐藥相關(guān)蛋白8抗體 0.2ml
AKR1C3 英文名稱: 醛固酮還原酶家族1成員C3抗體 0.2ml
Anti-AGER/Gold 金離子標(biāo)記的晚期糖基化終末產(chǎn)物特異性受體抗體Multi-class antibodies規(guī)格: 0.2ml
PGDS(Human Prostaglandin D Synthase) ELISA Kit 人素D合成酶Multi-class antibodies規(guī)格: 48T
Anti-Oct-4(Octamer-4) 胚胎干細(xì)胞關(guān)鍵蛋白抗體Multi-class antibodies規(guī)格: 0.1ml
Rhesus antibody Rh IDH1/Isocitrate dehydrogenase 異檸檬酸脫氫酶1抗體 規(guī)格 0.2ml
LPL(Mouse lipoprotein lipase)ELISA Kit 小鼠脂蛋白脂酶 96T
PCDH11Y 英文名稱: 原鈣粘附蛋白11Y抗體 0.2ml
C21orf2 英文名稱: 21號(hào)染色體開放閱讀框2抗體 0.2ml
Anti-Oct-4(Octamer-4) 胚胎干細(xì)胞關(guān)鍵蛋白抗體Multi-class antibodies規(guī)格: 0.1ml
Rappaport-VassiliadisMdeium
JM培養(yǎng)基基礎(chǔ) 250g 海博新產(chǎn)品
三糖鐵瓊脂(TSI) Triple Sugar Iron Agar 250 生化培養(yǎng)基,用于腸桿菌科細(xì)菌的生化反應(yīng)篩選(GB、SN標(biāo)準(zhǔn))
incubationmedia
NalidixicAcid
0.5%煌綠水溶液7ml/瓶添加到TTB溶液中
解磷細(xì)菌培養(yǎng)基 250g 用于解磷細(xì)菌的分離培養(yǎng)
腸毒素產(chǎn)毒培養(yǎng)基 250 用于金黃色葡萄球菌腸毒素產(chǎn)毒試驗(yàn)
0.5%葡萄糖肉湯培養(yǎng)基250g/瓶用于無菌檢查incubationmedia0.5%葡萄糖肉湯培養(yǎng)基250g/瓶用于無菌檢查
品紅亞瓊脂平板(9cm) 10個(gè)/包 用于飲用水,水源水中總大腸菌群的選擇性分離和確證
核受體相關(guān)因子-1抗體費(fèi)用 去氧膽酸鹽瓊脂(DC) 1000(g) incubation media 去氧膽酸鹽瓊脂(DC) 1000(g)
赫氏培養(yǎng)基 250(g) incubation media 赫氏培養(yǎng)基 250(g)
赫氏增菌液 250(g) incubation media 赫氏增菌液 250(g)
溶血瓊脂基礎(chǔ) 250(g) incubation media 溶血瓊脂基礎(chǔ) 250(g)
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 核受體相關(guān)因子-1抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)核受體相關(guān)因子-1抗體費(fèi)用 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。