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產品訂購信息：
英文名稱  Anti-SLC12A3/NCCT 

中文名稱   鈉氯離子轉運蛋白抗體品牌 

別    名  Na Cl symporter; Na-Cl symporter; NaCl electroneutral thiazide sensitive cotransporter; NCCT; S12A3_HUMAN; slc12a3; Solute carrier family 12 (sodium/chloride transporters) member 3; Solute carrier family 12 member 3; Thiazide sensitive Na Cl cotransporter; Thiazide sensitive sodium chloride cotransporter; Thiazide-sensitive sodium-chloride cotransporter; TSC.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Dog, Cow, Rabbit 

產品類型   一抗  

研究領域     心 細胞生物 通道蛋白 轉運蛋白

蛋白分子量  predicted molecular weight: 113kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SLC12A3/NCCT 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

鈉氯離子轉運蛋白抗體品牌 產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品介紹 Na-K-Cl cotransporters (NKCC) are channel proteins that aid in the transcellular movement of chloride across both secretory and absorptive epithelia. NKCC1 is expressed in muscle cells, neurons, and red blood cells. In the basolateral membrane of secretory epithelia, NKCC1 mediates active chloride secretion. The gene encoding human NKCC1 maps to chromosome 5q23.3. In mice, disruption of the NKCC1 gene leads to deafness and impaired balance. NKCC2 is specifically expressed in the kidney where it mediates active reabsorption of sodium chloride in the thick ascending limb of the loop of Henle. NKCC2 is sensitive to the clinically important diuretics furosemide and bumetanide. The gene encoding human NKCC2 maps to chromosome 15q15-q21 and mutations in this gene lead to Bartter’s syndrome, an inherited hypokalaemic alkalosis. NCCT is a thiazide-sensitive Na-Cl cotransporter that is primarily expressed in the distal convoluted tubule of the kidney where it accounts for a significant fraction of net renal sodium reabsorption. The gene for human NCCT map to chromosome 16q13. Mutations in the gene encoding NCCT cause Gitelman’s syndrome, a subset of Bartter’s syndrome.

Function : Electrically silent transporter system. Mediates sodium and chloride reabsorption.Subunit : Interacts with KLHL3.Subcellular Location : Membrane.Tissue Specificity : Predominant in kidney.Post-translational modifications : Ubiquitinated; ubiquitination is essential for regulation of endocytosis (By similarity). May be ubiquitinated by the BCR(KLHL3) complex.DISEASE : Defects in SLC12A3 are the cause of Gitelman syndrome (GS). GS is an autosomal recessive disorder characterized by hypokalemic alkalosis in combination with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. Patients are often asymptomatic or present transient periods of muscular weakness and tetany, usually accompanied by abdominal pain, vomiting and fever. The phenotype is highly heterogeneous in terms of age at onset and severity. Cardinal features such as hypocalciuria and hypomagnesemia might also change during the life cycle of a given patient. GS has overlapping features with Bartter syndrome.Similarity : Belongs to the SLC12A transporter family.

IL-6(Interleukin-6) 白介素6(白細胞介素-6)(多肽抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-Phospho-CD19 (Tyr531) 磷酸化CD19抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Pancreatic Amylase 胰淀粉酶抗體 規(guī)格 0.1ml

IL6R Kit Mouse 小鼠 IL6RA / CD126 ELISA配對抗體 ELISA

Trk A + B + C 英文名稱： 酪酸激酶A/B/C抗體 0.1ml

FAT3 英文名稱： 鈣粘蛋白15抗體 0.2ml

Anti-Phospho-CD19 (Tyr531) 磷酸化CD19抗體Multi-class antibodies規(guī)格： 0.1ml

Beta-TG ELISA Kit 大鼠β血小板球蛋白/β環(huán)蛋白Multi-class antibodies規(guī)格： 48T

Anti-ADRB3/ Beta3-AR /FITC 熒光素標記能受體β3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Fc γ RII a/CD32 免疫球蛋白G Fc段受體Ⅱ抗體 規(guī)格 0.1ml

APC(Mouse activated protein C) ELISA Kit 小鼠活化蛋白C 96T

MPS1 英文名稱： 單極紡錘體蛋白激酶1抗體 0.2ml

ABLIM3 英文名稱： 肌動蛋白結合蛋白LIM3抗體 0.2ml

Anti-ADRB3/ Beta3-AR /FITC 熒光素標記能受體β3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

MMP-2/Gelatinase A(Human Matrix metalloproteinase 2/Gelatinase A) ELISA kit 人基質金屬蛋白酶2/明膠酶AMulti-class antibodies規(guī)格： 48T

Anti-OPGL/ODF 骨保護蛋白配體抗體(破骨細胞分化因子)Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh ICOS/CD278 誘導協(xié)同刺激分子CD278抗體 規(guī)格 0.2ml

PAI ELISA Kit 大鼠纖溶酶原激活物抑制因子 96T

Protamine 2 英文名稱： 魚精蛋白2抗體 0.2ml

C17orf74 英文名稱： 17號染色體開放閱讀框74抗體 0.2ml

Anti-OPGL/ODF 骨保護蛋白配體抗體(破骨細胞分化因子)Multi-class antibodies規(guī)格： 0.1ml

志賀氏菌增菌肉湯250g用于志賀氏菌的選擇性增菌培養(yǎng)

Inorganicphosphorusbacteria

標準I號營養(yǎng)瓊脂 Standard I Nutrient Agar 250 標準細菌計數、含糖(MERCK方法)

Letheen肉湯基礎250g/瓶用于化妝品衛(wèi)生微生物檢測，每升培養(yǎng)基中需添加0204incubationmediaLetheen肉湯基礎250g/瓶用于化妝品衛(wèi)生微生物檢測，每升培養(yǎng)基中需添加0204

OxfordAgarBase

乳糖膽鹽培養(yǎng)基250g用于化妝品中糞大腸菌群的測定(GB標準)

菌種保存培養(yǎng)基 250(g) incubation media 菌種保存培養(yǎng)基 250(g)

TTC肉湯 ITC Broth 250克 分離培養(yǎng)耶爾森氏菌

M–肉湯250用于干燥食品和種子中沙門氏菌增菌培養(yǎng)(MERCK方法)incubationmediaM–肉湯250用于干燥食品和種子中沙門氏菌增菌培養(yǎng)(MERCK方法)

TetrathionateBrothBase

鈉氯離子轉運蛋白抗體品牌 Fraser肉湯增菌液FB1配套試劑  規(guī)格：  10支/盒  用途：  試劑A和試劑B各一支添加于225ml（026080）中配成FB1增菌液

李氏增菌液LB2凍干配套試劑  規(guī)格：  10支/盒  用途：  每支添加于200ml（026040）中配成LB2增菌液。

李氏增菌液LB1凍干配套試劑  規(guī)格：  10支/盒  用途：  每支添加于225ml（026040）中配成LB1增菌液。

EB增菌液凍干配套試劑  規(guī)格：  10支/盒  用途：  每支添加于225ml（026010）中配成EB增菌液

抗體的生物素化標記實驗要點：

1. 鈉氯離子轉運蛋白抗體品牌 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）鈉氯離子轉運蛋白抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。