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產(chǎn)品訂購信息：
英文名稱  Anti-NET1 

中文名稱   去甲腎上腺素轉(zhuǎn)運(yùn)蛋白/神經(jīng)遞質(zhì)去甲腎上腺素轉(zhuǎn)運(yùn)體抗體費(fèi)用 

別    名  NAT1; NET; NET; NET1; Norepinephrine transporter; SLC6A2; SLC6A5; SLC6A5; Sodium dependent noradrenaline transporter; Solute carrier family 6 (neurotransmitter transporter norepinephrine) member 5; Solute carrier family 6 member 2; Solute carrier family 6 member 2.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Cow, Horse 

產(chǎn)品類型   一抗  

研究領(lǐng)域    心 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 轉(zhuǎn)運(yùn)蛋白

蛋白分子量  predicted molecular weight: 65kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NET1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

去甲腎上腺素轉(zhuǎn)運(yùn)蛋白/神經(jīng)遞質(zhì)去甲腎上腺素轉(zhuǎn)運(yùn)體抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Catecholamine, a term used for the hormone adrenaline and its sequentially hydroxylated form noradrenaline, is involved in fight or flight responses. Noradrenaline is released from the post ganglionic sympathetic nerve endings and exerts its effects locally in the immediate vicinity of its release. In the CNS, noradrenaline is involved in a number of physiological responses including mood, sleep regulation, alertness and arousal, both cognitive and non-cognitive expression of behaviors, and control of the endocrine and autonomic nervous systems. Peripherally, noradrenaline is present in sympathetic nerve endings and has full control of the sympathetic nervous system. Noradrenaline released from pre-synaptic nerve terminals is reabsorbed (70-90%) by noradrenaline transporters and its biological effects are terminated. The noradrenaline transport via noradrenaline transporters is an active, Na+/Cl- dependent transport process mediated by noradrenaline transporters. Noradrenaline transporters constitute the primary mechanism for inactivation of synaptically released noradrenaline, are targets for multiple antidepressants and psychostimulants, and are deficient in affective and autonomic disorders. In rat brain, noradrenaline transporter is expressed in noradrenergic neuronal somata, axons and dendrites, and hippocampus and cortex, but is absent from epinephrine- and dopamine-containing neurons. At least 13 genetic variations have been reported in the noradrenaline transporter protein that affect noradrenaline re-uptake and concentrations in cerebrospinal fluid in humans. The association between these genetic variations in noradrenaline transporters and several psychiatric and cardiovascular disorders is just emerging. Recently, a single amino acid mutation (hNET-A457P) showed deficiency in noradrenaline transport in an orthostatic intolerance patient. Noradrenaline transporter protein consists of 617 amino acids and has 12 trans-membrane domains, a characteristic feature of many membrane associated solute transporters.

Function : Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.Subunit : Interacts with RHOA in its GTP- and GDP-bound states, and with CDC42 in its GTP-bound state. Interacts with the PDZ 1 domain of BAIAP1.Subcellular Location : Cytoplasm. Nucleus.Tissue Specificity : Widely expressed.Similarity : Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Database links : Swiss-Prot: Q7Z628.1

Anti-CK15/FITC 熒光素標(biāo)記細(xì)胞角蛋白15抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Integrin avb3/FITC 熒光素標(biāo)記整合素avb3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CCL6/C10 嗜酸粒細(xì)胞趨化蛋白CCL6抗體 規(guī)格 0.2ml

IL-4(Interleukin-4) 白介素4抗原 0.5mg

IKAP 英文名稱： IKK復(fù)合物相關(guān)蛋白抗體 0.1ml

Rhesus antibody Rh SP-D/PSPD 肺表面活性蛋白D抗體 規(guī)格 0.1ml

Anti-Integrin avb3/FITC 熒光素標(biāo)記整合素avb3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

LF/LTF(Mouse Lactoferrin) ELISA KIT 小鼠乳鐵傳遞蛋白/乳鐵蛋白Multi-class antibodies規(guī)格： 48T

Anti-Integrin Beta1/CD29(integrin beta 1)/FITC 熒光素標(biāo)記整合素β1蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Dynamin 2 酶動力蛋白2抗體 規(guī)格 0.1ml

ADV IgM III 腺病毒 IgM Ⅲ型(間接法二步法)

MOS 英文名稱： 原癌基因絲酸/蘇酸蛋白激酶MOS抗體 0.2ml

beta Adducin 英文名稱： 內(nèi)收蛋白抗體 0.1ml

Anti-Integrin Beta1/CD29(integrin beta 1)/FITC 熒光素標(biāo)記整合素β1蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

AMBP(Human alpha-1-microglobulin/bikunin precursor) ELISA Kit 人α1微球蛋白/bikunin前體Multi-class antibodies規(guī)格： 48T

Anti-OVA 雞卵白蛋白/卵清蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh IASPP 細(xì)胞凋亡ASPP家族的另一個成員抗體 規(guī)格 0.1ml

BP180-Ab(Human anti-Bullous Pemphigoid 180 antibody) ELISA Kit 人抗BP180抗體 96T

PLEKHM3 英文名稱： 血小板白細(xì)胞C激酶底物同源結(jié)構(gòu)域M3抗體 0.2ml

CLK2 英文名稱： 細(xì)胞分裂周期樣激酶2抗體 0.2ml

Anti-OVA 雞卵白蛋白/卵清蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

SodiumChlorideBloodAgarBase

HelicobacterPyloriMedium

GN 增菌液 Gram Negative Enrichment Broth 250 主要用于志賀氏菌的增菌培養(yǎng)，亦可用于沙門氏菌增菌培養(yǎng)(GB標(biāo)準(zhǔn))

結(jié)晶紫中性紅膽鹽葡萄糖瓊脂(VRBDA)250g/瓶腸道菌計數(shù)瓊脂，不含乳糖，用于腸道致病菌的選擇性分離和計數(shù)(SN、ISO)incubationmedia結(jié)晶紫中性紅膽鹽葡萄糖瓊脂(VRBDA)250g/瓶腸道菌計數(shù)瓊脂，不含乳糖，用于腸道致病菌的選擇性分離和計數(shù)(SN、ISO)

HB-PE自誘導(dǎo)培養(yǎng)基 250 用于基因工程菌大腸桿菌自誘導(dǎo)蛋白表達(dá)培養(yǎng)

ColiformChromogenicMedium

抗生素檢定培養(yǎng)基2號(高pH) Antibiotic Agar NO.2 用于林可霉素，克林霉素等效價測定

WS瓊脂 Wuhan Salmonella Agar 250克 分離沙門氏菌和志賀氏菌用

腸毒素產(chǎn)毒培養(yǎng)基250用于金黃色葡萄球菌腸毒素產(chǎn)毒試驗incubationmedia腸毒素產(chǎn)毒培養(yǎng)基250用于金黃色葡萄球菌腸毒素產(chǎn)毒試驗

DextroseSemisolidMedium

去甲腎上腺素轉(zhuǎn)運(yùn)蛋白/神經(jīng)遞質(zhì)去甲腎上腺素轉(zhuǎn)運(yùn)體抗體費(fèi)用 牛膽鹽  規(guī)格：  100g  用途：  細(xì)菌培養(yǎng)基的選擇性抑制劑

進(jìn)口細(xì)菌學(xué)瓊脂粉  規(guī)格：  10kg  用途：  培養(yǎng)基凝固劑

進(jìn)口細(xì)菌學(xué)瓊脂粉  規(guī)格：  500g  用途：  培養(yǎng)基凝固劑

技術(shù)瓊脂粉  規(guī)格：  500g  用途：  培養(yǎng)基凝固劑

抗體的生物素化標(biāo)記實驗要點(diǎn)：

1. 去甲腎上腺素轉(zhuǎn)運(yùn)蛋白/神經(jīng)遞質(zhì)去甲腎上腺素轉(zhuǎn)運(yùn)體抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）去甲腎上腺素轉(zhuǎn)運(yùn)蛋白/神經(jīng)遞質(zhì)去甲腎上腺素轉(zhuǎn)運(yùn)體抗體費(fèi)用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。