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★磷酸化細胞核受體Rev-Erbα抗體品牌 分子生物學：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構(gòu)建、細胞RNAi技術(shù)等等。

實驗流程：

磷酸化細胞核受體Rev-Erbα抗體品牌 英文名稱  Anti-Phospho-NR1D1(Ser55/59)

中文名稱  磷酸化細胞核受體Rev-Erbα抗體品牌 

別    名 EAR-1; EAR1; ERBA-RELATED 1; hRev; Nr1d1; NR1D1_HUMAN; Nuclear Receptor Rev-ErbA Alpha; Nuclear receptor subfamily 1 group D member 1; Orphan nuclear receptor NR1D1; Rev erbAalpha; Rev erbalpha; Rev-erbA-alpha; Rev-ErbAalpha; REV-ERBalpha; THRA1; THRAL; Thyroid hormone receptor alpha-like; Thyroid hormone receptor, alpha like; V-erbA related protein EAR-1; V-erbA-related protein 1.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Cow, Sheep

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    細胞生物 免疫學 染色質(zhì)和核信號 神經(jīng)生物學 信號轉(zhuǎn)導 生長因子和激素 表觀遺傳學

蛋白分子量  predicted molecular weight: 67kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human REV-ERB alpha around the phosphorylation site of Ser55/59

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化細胞核受體Rev-Erbα抗體品牌 產(chǎn)品應用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 NR1D1, a NR1 Thyroid Hormone-Like Receptor, is encoded by the same genomic locus as, but transcribed from the opposite strand of, Thyroid Hormone Receptor Alpha (TR Alpha). NR1D1 is a target of Nuclear Receptor ROR Alpha and a transcription regulator that has been shown to affect myocyte differentiation, adipogenesis, and lipoprotein metabolism. Mice lacking NR1D1 show abnormal postnatal cerebellar development. NR1D1 expression has been documented in human skeletal muscle and a variety of mouse and rat tissues. ESTs have been isolated from human tissue libraries, including cancerous adrenal, blood, brain, breast, colon, duodenum, fetus, head/neck, kidney, lung, skeletal muscle, skin, synovium, uterus, normal brain, breast, colon, eye, heart, pancreas, pituitary, prostate, skeletal muscle, skin, testis and thyroid.

Function : Functions as a constitutive transcriptional repressor. In collaboration with SP1, activates GJA1 transcription (By similarity). Possible receptor for triiodothyronine.Subunit : Interacts with C1D and NR2E3 (By similarity). Interacts with SP1 (By similarity).Subcellular Location : Nucleus (Potential).Tissue Specificity : Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.Similarity : Belongs to the nuclear hormone receptor family. NR1 subfamily.Contains 1 nuclear receptor DNA-binding domain.Database links : UniProtKB/Swiss-Prot: P20393.11.細胞核受體Rev-Erbα是生物鐘系統(tǒng)的重要組成部分,不僅是生物鐘基因而且是生物鐘；2.Rev-Erbα。

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