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中文名稱(chēng)  核抑制蛋白磷酸酶1抗體規(guī)格 

英文名稱(chēng)  Anti-NIPP1/ARD1

別    名 Activator of RNA decay; ARD 1; ARD1; Homolog of E.coli RNase E; NIPP 1; NIPP1; Nuclear inhibitor of protein phosphatase 1; Nuclear subunit of PP1; PPP1R8; Protein phosphatase 1 regulatory inhibitor subunit 8; Protein phosphatase 1 regulatory subunit 8; RNase E.

產(chǎn)品屬性：

核抑制蛋白磷酸酶1抗體規(guī)格 濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Guinea Pig, Daniorerio

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域     免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 38kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human NIPP1(25-75aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

核抑制蛋白磷酸酶1抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Inhibitor subunit of the major nuclear protein phosphatase 1 (PP1). It has RNA binding activity but does not cleave RNA and may target PP1 to RNA associated substrates. May also be involved in pre mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.

Function : Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.Subunit : Interacts with phosphorylated CDC5L, SF3B1 and MELK. Interacts with EED, in a nucleic acid-stimulated manner. Part of a complex consisting of PPP1R8, EED, HDAC2 and PP-1. Part of the spliceosome. Interacts with PPP1CA, PPP1CB and PPP1CC.Subcellular Location : Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear. Isoform Gamma: Cytoplasm. Note=Found mainly in the cytoplasm.Tissue Specificity : Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.Post-translational modifications : May be inactivated by phosphorylation on Ser-199 or Ser-204. Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.Similarity : Contains 1 FHA domain.NIPP1是一種普遍存在于細(xì)胞核中的蛋白，它在剪接體的裝配中發(fā)揮著重要作用，主要體現(xiàn)在細(xì)胞的分化，增值及遷移中。經(jīng)研究，目前NIPP1在、,以及中有較高頻率的表達(dá),其中中的表達(dá)頻率*,達(dá)到70%,遠(yuǎn)高于其他組織。表明ARD1蛋白的高表達(dá)可能是組織的一個(gè)標(biāo)志。

實(shí)驗(yàn)流程：

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優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線(xiàn)技術(shù)咨詢(xún)。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂(yōu)。

技術(shù)外包服務(wù):

★核抑制蛋白磷酸酶1抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

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