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產(chǎn)品訂購信息：
英文名稱  Anti-NF2/Neurofibromin 2 

中文名稱   2型神經(jīng)抗體說明書 

別    名  neurofibromatosis type 2; CAN; BANF; bilateral acoustic neuroma; moesin ezrin radixin like protein; Moesin ezrin radizin like protein; merlin; Neurofibromatosis 2; ACN; Bilateral acoustic neuroma; MERL_HUMAN; Merlin; Moesin ezrin radizin like; Moesin-ezrin-radixin-like protein; Neurofibromatosis type 2; Neurofibromin-2; NF 2; Nf2; Neurofibromatosis2; Neurofibromin 2; Neurofibromin2; Schwannomerlin; NF-2; SCH; Schwannomin.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 70kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NF2 C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

2型神經(jīng)抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Neurofibromin 2 (NF2) is a tumor suppressor gene encoding the protein Merlin. Merlin is closely related to the ERM (ezrin, radixin, moesin) family of proteins. The precise funtion of Merlin is not clear. It is thought to provide a link between the actin cytoskeleten and membrane associated proteins, playing a role in transduction of extracellular signals. It has been implicated in cell proliferation and cellular motility.

Mutations in the NF2 gene cause neurofibromatosis type II, a condition characterised by the development of tumors in the central nervous system.Function : Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.Subunit : Interacts with SLC9A3R1, HGS and AGAP2. Interacts with LAYN (By similarity). Interacts with SGSM3. Interacts (via FERM domain) with MPP1. Interacts with WWC1. Interacts with the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. The unphosphorylated form interacts (via FERM domain) with VPRBP/DCAF1.Subcellular Location : Isoform 1: Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with VPRBP in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.Isoform 7: Cytoplasm, perinuclear region. Cytoplasmic granule. Note=Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 7 is absent from ruffling membranes and filopodia.Isoform 9: Cytoplasm, perinuclear region. Cytoplasmic granule. Note=Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia.Isoform 10: Nucleus. Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, perinuclear region. Cytoplasmic granule. Cytoplasm, cytoskeleton. Note=In a fibroblastic cell line, isoform 10 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia.Tissue Specificity : Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.Post-translational modifications : Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail.Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.DISEASE : Neurofibromatosis 2 (NF2) [MIM:101000]: Genetic disorder characterized by bilateral vestibular schwannomas (formerly called acoustic neuromas), schwannomas of other cranial and peripheral nerves, meningiomas, and ependymomas. It is inherited in an autosomal dominant fashion with full penetrance. Affected individuals generally develop symptoms of eighth-nerve dysfunction in early adulthood, including deafness and balance disorder. Although the tumors of NF2 are histologically benign, their anatomic location makes management difficult, and patients suffer great morbidity and mortality. Note=The disease is caused by mutations affecting the gene represented in this entry.Schwannomatosis (SCHWA) [MIM:162091]: Schwannomas are benign tumors of the peripheral nerve sheath that usually occur singly in otherwise normal individuals. Multiple schwannomas in the same individual suggest an underlying tumor-predisposition syndrome. The most common such syndrome is NF2. The hallmark of NF2 is the development of bilateral vestibular-nerve schwannomas; but two-thirds or more of all NF2-affected individuals develop schwannomas in other locations, and dermal schwannomas may precede vestibular tumors in NF2-affected children. There have been several reports of individuals with multiple schwannomas who do not show evidence of vestibular schwannoma. Clinical report suggests that schwannomatosis is a clinical entity distinct from other forms of neurofibromatosis. Note=The disease is caused by mutations affecting the gene represented in this entry.Mesothelioma, malignant (MESOM) [MIM:156240]: An aggressive neoplasm of the serosal lining of the chest. It appears as broad sheets of cells, with some regions containing spindle-shaped, sarcoma-like cells and other regions showing adenomatous patterns. Pleural mesotheliomas have been linked to exposure to asbestos. Note=The disease may be caused by mutations affecting the gene represented in this entry.Similarity : Contains 1 FERM domain.Database links : UniProtKB/Swiss-Prot: P35240.1Ⅱ型神經(jīng)(neurofibromatosisⅡ,NF2)又稱merlin蛋白在細(xì)胞與細(xì)胞的信號傳遞中起著關(guān)鍵作用，可能與其它類型也有關(guān)聯(lián)。

P311 protein 相關(guān)蛋白(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-CD54/ICAM-1 細(xì)胞間粘附分子-1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh P38 MAPK(Phospho-Thr180/Tyr182) 磷酸化-原活化蛋白激酶p38抗體(P-p38 MAPK) 規(guī)格 0.1ml

IL1B Kit Human 人 IL-1 beta / IL1B / IL-1F2 ELISA配對抗體 ELISA

TUB 1 英文名稱： TUB蛋白抗體 0.2ml

COBL 英文名稱： -綜合征相關(guān)COBL蛋白抗體 0.1ml

Anti-CD54/ICAM-1 細(xì)胞間粘附分子-1抗體Multi-class antibodies規(guī)格： 0.1ml

Resistin ELISA Kit 大鼠抵抗素Multi-class antibodies規(guī)格： 48T

Anti-ASPP2/FITC 熒光素標(biāo)記P53凋亡刺激蛋白2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FAM101A FAM101A蛋白抗體 規(guī)格 0.2ml

RBP ELISA Kit 大鼠視黃醇結(jié)合蛋白 96T

MMP-1 英文名稱： 基質(zhì)金屬蛋白酶-1抗體 0.1ml

AIPL1 英文名稱： 遺傳性相關(guān)蛋白AIPL1抗體 0.1ml

Anti-ASPP2/FITC 熒光素標(biāo)記P53凋亡刺激蛋白2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-GRO Alpha/FITC 熒光素標(biāo)記兔抗生長調(diào)節(jié)致癌基因α抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-p53BP1/53BP1/FITC 熒光素標(biāo)記兔抗人、大、小鼠p53結(jié)合蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh beta Bax/BCL2 associated X protein BCL2相關(guān)X蛋白抗體 規(guī)格 0.2ml

AMH/MIS Muellerian抑制因子抗原 1mg

phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) 英文名稱： 磷酸化糖原合酶激酶3α/β抗體 0.1ml

Rhesus antibody Rh RanBP3 RAN結(jié)合蛋白3抗體 規(guī)格 0.2ml

Anti-p53BP1/53BP1/FITC 熒光素標(biāo)記兔抗人、大、小鼠p53結(jié)合蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

水楊素分裝，與HB使用

FRASER Listeria Selective　Supplement 弗雷澤李斯特菌選擇添加劑 1.10399.0001 MERCK默克 incubation media FRASER Listeria Selective　Supplement 弗雷澤李斯特菌選擇添加劑 1.10399.0001 MERCK默克

改良山梨醇麥康凱瓊脂基礎(chǔ)(CT-SMAC) 250g 用于大腸埃希氏菌0157:H7/NM的選擇性分離培養(yǎng)(GB/T4789.36-2008)。

C57BL/6小鼠骨髓間質(zhì)干細(xì)胞成軟骨誘導(dǎo)分化完全培養(yǎng)基C57BL/6mousebonemarrowmesenchymalstemcellsintochondrocytesinduceddifferentiationofcompletemedium

NBB培養(yǎng)基 250g 用于啤酒中厭氧菌檢測

xtroseBrothMedium

李氏菌增菌肉湯(LB1,LB2)基礎(chǔ) Listeria Enrichment Broth Base 添加奈啶酮酸，黃素，即為LB1，LB2，用于李氏菌二步增菌(GB標(biāo)準(zhǔn))

WILKINS-CHALGREN厭氧菌瓊脂 WILKINS-CHALGREN Agar 250克 厭氧菌的分離培養(yǎng)

吡哆醇Y培養(yǎng)基瓶用于吡哆醇測定incubationmedia吡哆醇Y培養(yǎng)基瓶用于吡哆醇測定

腦心浸萃液肉湯 250g 用于營養(yǎng)苛求細(xì)菌的增菌培養(yǎng)

2型神經(jīng)抗體說明書 苯 生化鑒定管  規(guī)格：  20支/盒  用途：  細(xì)菌生化鑒定

葡萄糖酸鹽生化鑒定管  規(guī)格：  20支/盒  用途：  細(xì)菌生化鑒定

西蒙氏枸椽酸鹽生化鑒定管  規(guī)格：  20支/盒  用途：  細(xì)菌生化鑒定

鹽生化鑒定管  規(guī)格：  20支/盒  用途：  細(xì)菌生化鑒定

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 2型神經(jīng)抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）2型神經(jīng)抗體說明書 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。