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抗體的生物素化標記實驗要點：

1. T細胞激活核轉(zhuǎn)錄因子4抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-NF-ATc4 

中文名稱   T細胞激活核轉(zhuǎn)錄因子4抗體規(guī)格 

別    名  NFATC4 cytoplasmic 4; NF ATc4; NF-AT3; NF-ATc4; NFAC4_HUMAN; NFAT3; NFATc4; Nuclear factor of activated T cells cytoplasmic 4; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4; Nuclear factor of activated T-cells; nuclear factor of activated T-cells cytoplasmic 4; Nuclear factor of activated T-cells cytoplasmic calcineurin-dependent 4; T cell transcription factor NFAT3; T-cell transcription factor NFAT3.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 淋巴細胞 t-淋巴細胞 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 95kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NF-ATc4 (475-525aa) 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

T細胞激活核轉(zhuǎn)錄因子4抗體規(guī)格 產(chǎn)品應(yīng)用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The product of this gene is a member of the nuclearfactors of activated T cells DNA-binding transcription complex.This complex consists of at least two components: a preexistingcytosolic component that translocates to the nucleus upon T cellreceptor (TCR) stimulation and an inducible nuclear component.Other members of this family of nuclear factors of activated Tcells also participate in the formation of this complex. Theproduct of this gene plays a role in the inducible expression ofcytokine genes in T cells, especially in the induction of the IL-2and IL-4. Alternatively spliced transcript variants encodingdifferent isoforms have been noted for this gene.

Function : Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.Subunit : Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. Interacts with CREBBP, GATA4, IRAK1, MAPK8, MAPK9 and RPS6KA3.Subcellular Location : Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.Tissue Specificity : Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.Post-translational modifications : Phosphorylated by NFATC-kinases; dephosphorylated bycalcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1,MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and onSer-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.Ubiquitinated, leading to its degradation by the proteasomeand reduced transcriptional activity. Ubiquitination and reductionin transcriptional activity can be further facilitated throughGSK3B-dependent phosphorylation. Polyubiquitin linkage is mainlythrough 'Lys-48'.Similarity : Contains 1 IPT/TIG domain.Contains 1 RHD (Rel-like) domain.Database links : UniProtKB/Swiss-Prot: Q14934.2

抗體的鑒定：

1）T細胞激活核轉(zhuǎn)錄因子4抗體規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

CD133 Anti-gen 造血干細胞抗原(多肽抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-CCL11/Eotaxin 1 嗜酸粒細胞趨化蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh PCDHA3 原鈣粘附蛋白α3抗體 規(guī)格 0.2ml

IL18R1 Kit Human 人 IL18R1 / CD218a ELISA配對抗體 ELISA

Thyroxine Binding Globulin 英文名稱： 甲狀腺素結(jié)合球蛋白抗體 0.2ml

CD10 英文名稱： CD10抗體 0.1ml

Anti-CCL11/Eotaxin 1 嗜酸粒細胞趨化蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

CA(Mouse carbonic anhydrase) ELISA Kit 小鼠酶Multi-class antibodies規(guī)格： 48T

Anti-TNFRSF13B/TNFRSF14B/TACI/FITC 熒光素標記壞死因子受體超家族成員13B抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Donkey Anti-human IgG 驢抗人IgG 規(guī)格 1mg

5-HT (Rabbit 5-Hydroxytryptamine) ELISA Kit 兔子5羥色胺 96T

MLX 英文名稱： 轉(zhuǎn)化因子樣蛋白4抗體 0.2ml

ARSB 英文名稱： 芳基酯酶B抗體 0.2ml

Anti-TNFRSF13B/TNFRSF14B/TACI/FITC 熒光素標記壞死因子受體超家族成員13B抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-IL6R Alpha /FITC 熒光素標記白細胞介素6受體α抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-p58ipk/FITC 熒光素標記p58ipk抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh BECN1L1 Bcl-2同源結(jié)構(gòu)域樣蛋白1抗體 規(guī)格 0.2ml

Caspase-1 (ICE; CASP-1;P45) 天冬酸-胱酸特異性蛋白酶家族(抗原) 0.5mg

GPR85 英文名稱： G蛋白偶聯(lián)受體GPR85蛋白抗體 0.2ml

Rhesus antibody Rh RAG1/RNF74 環(huán)指蛋白74抗體(重組激活基因1) 規(guī)格 0.2ml

Anti-p58ipk/FITC 熒光素標記p58ipk抗體IgGMulti-class antibodies規(guī)格： 0.2ml

牛肝顆粒同肝湯配套使用

Fraser Broth Base incubation media Fraser Broth Base

變形桿菌 支/瓶

啶酮酸5.0mg/支*5每支添加于225mlHBJ005中制成LB1肉湯

玫瑰紅瓊脂 250g 用于霉菌、酵母菌計數(shù)

歐洲藥典培養(yǎng)基(EP標準)

李斯特菌顯色培養(yǎng)基 1000 mL/瓶 incubation media 李斯特菌顯色培養(yǎng)基 1000 mL/瓶

李氏增菌液LB1凍干配套試劑 10支 每支添加于225ml(026040)中配成LB1增菌液。

培養(yǎng)基PMediumP(Reinforedmediumforclostridia)用于梭菌的增菌培養(yǎng)和計數(shù)。

APTAgar

T細胞激活核轉(zhuǎn)錄因子4抗體規(guī)格 生化鑒定管  規(guī)格：  20支/盒  用途：  細菌生化鑒定

基酸脫羧酶對照生化鑒定管  規(guī)格：  20支/盒  用途：  細菌生化鑒定

賴酸脫羧酶生化鑒定管  規(guī)格：  20支/盒  用途：  細菌生化鑒定

鳥酸脫羧酶生化鑒定管  規(guī)格：  20支/盒  用途：  細菌生化鑒定