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實(shí)驗流程：

中分子量神經(jīng)絲蛋白抗體費(fèi)用 英文名稱  Anti-NF-M/Neurofilament M

中文名稱  中分子量神經(jīng)絲蛋白抗體費(fèi)用 

別    名 Neurofilament medium polypeptide; 160 kDa neurofilament protein; neurofilament 3; Nefm; 150kDa medium; NEF 3; NEF3; NEFM; Neurofilament 3; Neurofilament medium polypeptide; Neurofilament protein medium; Neurofilament triplet M protein; Neurofilament3; NF M; NF160; Neurofilament-M; Neurofilament M; NFM.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Cow

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué)

蛋白分子量  predicted molecular weight: 102kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human NF-M N-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

中分子量神經(jīng)絲蛋白抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Function : Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.Post-translational modifications : There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.Similarity : Belongs to the intermediate filament family.神經(jīng)生物學(xué)相關(guān)蛋白（Neurobiology）中分子量神經(jīng)絲蛋白,簡稱NF-L,分子量為160kDa ；NF-L的聚集與神經(jīng)退行性疾病的機(jī)理相關(guān)，如運(yùn)動神經(jīng)元的降解等。神經(jīng)纖絲蛋白的功能是提供彈性使神經(jīng)纖維易于伸展和防止斷裂。神經(jīng)絲是中間纖維的一種重要類型又稱神經(jīng)微絲蛋白，特異地在神經(jīng)細(xì)胞內(nèi)表達(dá)，并在軸突內(nèi)相互平行排列成束.　哺乳動物的神經(jīng)絲由3種蛋白組成：低分子量神經(jīng)絲蛋白，簡稱NF-L；分子量為68kDa；中分子量神經(jīng)絲蛋白，簡稱NF-M；分子量為160kDa；高分子量神經(jīng)絲蛋白，簡稱NF-H，分子量為200kDa.

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